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DOI10.1016/j.aca.2006.05.037
Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE)
Koivunen, Marja E.; Dettmer, Katja; Vermeulen, Roel; Bakke, Berit; Gee, Shirley J.; Hammock, Bruce D.
通讯作者Hammock, Bruce D.
来源期刊ANALYTICA CHIMICA ACTA
ISSN0003-2670
EISSN1873-4324
出版年2006
卷号572期号:2页码:180-189
英文摘要

Elimination of interfering substances in urine by solid phase extraction (SPE) prior to analysis resulted in 10-fold improvement in the sensitivity of atrazine mercapturate (AM) enzyme-linked immunosorbent assay (ELISA) compared to previous reports. Of the two tested SPE systems, Oasis (R) HLB and MCX, the mixed-mode MCX gave good recoveries (82%) of AM in spiked samples measured by ELISA, whereas the reverse-phase HLB phase was not compatible with the immunochemical method. At relatively high concentrations of urinary AM (> 20 ng mL(-1)), sample dilution was effective enough for the elimination of interfering substances. The new liquid chromatography-mass spectrometry (LC-MS) method developed for AM utilizes online-SPE with Oasis (R) HLB, column switching and a stable-isotope internal standard. The limit of quantification (0.05 ng mL(-1)) indicates improved sensitivity compared with most previously published LC-MS methods for AM. Validation of all three methods, LC-MS, ELISA + SPE and ELISA + dilution with spiked urine samples showed good correlation between the known and measured concentrations with R-2 values of 0.996, 0.957 and 0.961, respectively. When a set (n = 70 plus 12 blind duplicates) of urine samples from farmers exposed to atrazine was analyzed, there was a good agreement (R-2 = 0. 917) between the log normalized data obtained by ELISA + SPE and LC-MS. High correlation among the data obtained by the two tested methods and the LC-MS method by the Center of Disease Control and Prevention (CDC), together with low variability among the blind duplicates, suggests that both methods reported here would be suitable for the analysis of urinary AM as a biomarker for human exposure of atrazine. (c) 2006 Elsevier B.V. All rights reserved.


英文关键词atrazine mercapturate enzyme-linked immunosorbent assay (ELISA) solid phase extraction (SPE) online solid phase extraction liquid chromatography-mass spectrometry (LC-MS) human biomonitoring
类型Article
语种英语
国家USA ; Norway
收录类别SCI-E
WOS记录号WOS:000239234900003
WOS关键词EXPOSURE BIOMARKERS TRANS ; S-PHENYLMERCAPTURIC ACID ; LOOP CLEANUP DEVICE ; PESTICIDE METABOLITES ; MUCONIC ACID ; QUANTIFICATION ; APPLICATORS ; HERBICIDES ; SYSTEM
WOS类目Chemistry, Analytical
WOS研究方向Chemistry
来源机构University of California, Davis
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/150682
作者单位(1)Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA;(2)Univ Calif Davis, Ctr Canc, Davis, CA 95616 USA;(3)NCI, Div Canc Epidemiol & Genet, DHHS, NIH, Rockville, MD USA;(4)Natl Inst Occupat Hlth, Oslo, Norway
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Koivunen, Marja E.,Dettmer, Katja,Vermeulen, Roel,et al. Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE)[J]. University of California, Davis,2006,572(2):180-189.
APA Koivunen, Marja E.,Dettmer, Katja,Vermeulen, Roel,Bakke, Berit,Gee, Shirley J.,&Hammock, Bruce D..(2006).Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE).ANALYTICA CHIMICA ACTA,572(2),180-189.
MLA Koivunen, Marja E.,et al."Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE)".ANALYTICA CHIMICA ACTA 572.2(2006):180-189.
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