Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.aca.2006.05.037 |
Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE) | |
Koivunen, Marja E.; Dettmer, Katja; Vermeulen, Roel; Bakke, Berit; Gee, Shirley J.; Hammock, Bruce D. | |
通讯作者 | Hammock, Bruce D. |
来源期刊 | ANALYTICA CHIMICA ACTA
![]() |
ISSN | 0003-2670 |
EISSN | 1873-4324 |
出版年 | 2006 |
卷号 | 572期号:2页码:180-189 |
英文摘要 | Elimination of interfering substances in urine by solid phase extraction (SPE) prior to analysis resulted in 10-fold improvement in the sensitivity of atrazine mercapturate (AM) enzyme-linked immunosorbent assay (ELISA) compared to previous reports. Of the two tested SPE systems, Oasis (R) HLB and MCX, the mixed-mode MCX gave good recoveries (82%) of AM in spiked samples measured by ELISA, whereas the reverse-phase HLB phase was not compatible with the immunochemical method. At relatively high concentrations of urinary AM (> 20 ng mL(-1)), sample dilution was effective enough for the elimination of interfering substances. The new liquid chromatography-mass spectrometry (LC-MS) method developed for AM utilizes online-SPE with Oasis (R) HLB, column switching and a stable-isotope internal standard. The limit of quantification (0.05 ng mL(-1)) indicates improved sensitivity compared with most previously published LC-MS methods for AM. Validation of all three methods, LC-MS, ELISA + SPE and ELISA + dilution with spiked urine samples showed good correlation between the known and measured concentrations with R-2 values of 0.996, 0.957 and 0.961, respectively. When a set (n = 70 plus 12 blind duplicates) of urine samples from farmers exposed to atrazine was analyzed, there was a good agreement (R-2 = 0. 917) between the log normalized data obtained by ELISA + SPE and LC-MS. High correlation among the data obtained by the two tested methods and the LC-MS method by the Center of Disease Control and Prevention (CDC), together with low variability among the blind duplicates, suggests that both methods reported here would be suitable for the analysis of urinary AM as a biomarker for human exposure of atrazine. (c) 2006 Elsevier B.V. All rights reserved. |
英文关键词 | atrazine mercapturate enzyme-linked immunosorbent assay (ELISA) solid phase extraction (SPE) online solid phase extraction liquid chromatography-mass spectrometry (LC-MS) human biomonitoring |
类型 | Article |
语种 | 英语 |
国家 | USA ; Norway |
收录类别 | SCI-E |
WOS记录号 | WOS:000239234900003 |
WOS关键词 | EXPOSURE BIOMARKERS TRANS ; S-PHENYLMERCAPTURIC ACID ; LOOP CLEANUP DEVICE ; PESTICIDE METABOLITES ; MUCONIC ACID ; QUANTIFICATION ; APPLICATORS ; HERBICIDES ; SYSTEM |
WOS类目 | Chemistry, Analytical |
WOS研究方向 | Chemistry |
来源机构 | University of California, Davis |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/150682 |
作者单位 | (1)Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA;(2)Univ Calif Davis, Ctr Canc, Davis, CA 95616 USA;(3)NCI, Div Canc Epidemiol & Genet, DHHS, NIH, Rockville, MD USA;(4)Natl Inst Occupat Hlth, Oslo, Norway |
推荐引用方式 GB/T 7714 | Koivunen, Marja E.,Dettmer, Katja,Vermeulen, Roel,et al. Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE)[J]. University of California, Davis,2006,572(2):180-189. |
APA | Koivunen, Marja E.,Dettmer, Katja,Vermeulen, Roel,Bakke, Berit,Gee, Shirley J.,&Hammock, Bruce D..(2006).Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE).ANALYTICA CHIMICA ACTA,572(2),180-189. |
MLA | Koivunen, Marja E.,et al."Improved methods for urinary atrazine mercapturate analysis - Assessment of an enzyme-linked immunosorbent assay (ELISA) and a novel liquid chromatography-mass spectrometry (LC-MS) method utilizing online solid phase extraction (SPE)".ANALYTICA CHIMICA ACTA 572.2(2006):180-189. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。