Arid
DOI10.1073/pnas.0406798102
Development and evaluation of a microdevice for amino acid biomarker detection and analysis on Mars
Skelley, AM; Scherer, JR; Aubrey, AD; Grover, WH; Ivester, RHC; Ehrenfreund, P; Grunthaner, FJ; Bada, JL; Mathies, RA
通讯作者Mathies, RA
来源期刊PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
出版年2005
卷号102期号:4页码:1041-1046
英文摘要

The Mars Organic Analyzer (MOA), a microfabricated capillary electrophoresis (CE) instrument for sensitive amino acid biomarker analysis, has been developed and evaluated. The microdevice consists of a four-wafer sandwich combining glass CE separation channels, microfabricated pneumatic membrane valves and pumps, and a nanoliter fluidic network. The portable MOA instrument integrates high voltage CE power supplies, pneumatic controls, and fluorescence detection optics necessary for field operation. The amino acid concentration sensitivities range from micromolar to 0.1 nM, corresponding to part-per-trillion sensitivity. The MOA was first used in the lab to analyze soil extracts from the Atacama Desert, Chile, detecting amino acids ranging from 10-600 parts per billion. Field tests of the MOA in the Panoche Valley, CA, successfully detected amino acids at 70 parts per trillion to 100 parts per billion in jarosite, a sulfate-rich mineral associated with liquid water that was recently detected on Mars. These results demonstrate the feasibility of using the MOA to perform sensitive in situ amino acid biomarker analysis on soil samples representative of a Mars-like environment.


英文关键词amino acid analysis astrobiology capillary electrophoresis microfabrication
类型Article
语种英语
国家USA ; Netherlands
收录类别SCI-E
WOS记录号WOS:000226617900017
WOS关键词MARTIAN METEORITE ALH84001 ; EXTRATERRESTRIAL EXPLORATION ; ATACAMA DESERT ; LIFE ; SEARCH ; ENANTIOMERS ; BACTERIA ; DEVICES ; SAMPLES ; SOILS
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
来源机构University of California, Berkeley
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/150240
作者单位(1)Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA;(2)Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA;(3)Leiden Univ, Astrobiol Lab, NL-2300 RA Leiden, Netherlands;(4)CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA
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Skelley, AM,Scherer, JR,Aubrey, AD,et al. Development and evaluation of a microdevice for amino acid biomarker detection and analysis on Mars[J]. University of California, Berkeley,2005,102(4):1041-1046.
APA Skelley, AM.,Scherer, JR.,Aubrey, AD.,Grover, WH.,Ivester, RHC.,...&Mathies, RA.(2005).Development and evaluation of a microdevice for amino acid biomarker detection and analysis on Mars.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,102(4),1041-1046.
MLA Skelley, AM,et al."Development and evaluation of a microdevice for amino acid biomarker detection and analysis on Mars".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 102.4(2005):1041-1046.
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