干旱区生态环境知识资源中心(Arid): Purification and characterisation of extracellular protease produced by Clostridium sp from Schirmacher oasis, Antarctica

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DOI	10.1016/j.enzmictec.2005.01.011
	Purification and characterisation of extracellular protease produced by Clostridium sp from Schirmacher oasis, Antarctica
	Alam, SI; Dube, S; Reddy, GSN; Btlattacharya, BK; Shivaji, S; Singh, L
通讯作者	Singh, L
来源期刊	ENZYME AND MICROBIAL TECHNOLOGY
ISSN	0141-0229
EISSN	1879-0909
出版年	2005
卷号	36 期号:5-6 页码:824-831
英文摘要	One anaerobic, proteolytic bacterium isolated from Schirmacher oasis, Antarctica was characterised taxonomically. Based on morphology, biochemical characteristics and 16S rRNA sequence the isolate was identified as Clostridium species with closest similarity with Clostridium subterminale. Isolate was psychrotrophic forming maximum cell mass between 5 and 10 degrees C and produced extracellular protease. Growth was observed in the pH range of 6.5-8.5 with optimum at pH 8. Protease was purified 12.7-fold with a total yield of 26.2%. Effect of temperature, pH and salt concentration on enzyme activity were studied. Protease was found to be a serine-type metaloenzyme, which is active in a broad range of pH. It was moderately thermolabile and resistant to SDS. Enzyme kinetics revealed a tendency to decrease K. with increase in temperature for casein substrate. (c) 2005 Elsevier Inc. All rights reserved.
英文关键词	Clostridium psychrotroph Antarctica protease anaerobe
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000227781000029
WOS关键词	SP-NOV ; PROTEOLYTIC BACTERIUM ; COLD ADAPTATION ; LOW-TEMPERATURE ; ACE LAKE ; MICROORGANISMS ; AMYLASE ; ENZYMES ; ICE
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/148949
作者单位	(1)Def Res & Dev Estab, Div Biotechnol, Gwalior 474002, India;(2)Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India;(3)Def Res & Dev Estab, Div Biochem, Gwalior 474002, India
推荐引用方式 GB/T 7714	Alam, SI,Dube, S,Reddy, GSN,et al. Purification and characterisation of extracellular protease produced by Clostridium sp from Schirmacher oasis, Antarctica[J],2005,36(5-6):824-831.
APA	Alam, SI,Dube, S,Reddy, GSN,Btlattacharya, BK,Shivaji, S,&Singh, L.(2005).Purification and characterisation of extracellular protease produced by Clostridium sp from Schirmacher oasis, Antarctica.ENZYME AND MICROBIAL TECHNOLOGY,36(5-6),824-831.
MLA	Alam, SI,et al."Purification and characterisation of extracellular protease produced by Clostridium sp from Schirmacher oasis, Antarctica".ENZYME AND MICROBIAL TECHNOLOGY 36.5-6(2005):824-831.