干旱区生态环境知识资源中心(Arid): Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp P9

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DOI	10.1016/j.enzmictec.2004.11.017
	Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp P9
	Dhaked, RK; Alam, SI; Dixit, A; Singh, L
通讯作者	Singh, L
来源期刊	ENZYME AND MICROBIAL TECHNOLOGY
ISSN	0141-0229
EISSN	1879-0909
出版年	2005
卷号	36 期号:7 页码:855-861
英文摘要	A psychrotolerant Bacillus sp. from Antarctica produced an alkaline phosphatase in the culture supernatant. The strain showed 98.4% 16s rDNA sequence identity with Bacillus sphaericus. The 76 kDa protein was purified 11.1-fold showing alkaline phosphomonoesterase activity. Enzyme was optimally produced at 25 degrees C and pH 7.0. This cold active alkaline phosphatase is heat labile and gets completely inactivated at 60 degrees C in 50 min and is active in broad pH range. (c) 2005 Elsevier Inc. All rights reserved.
英文关键词	alkaline phosphatase psych rotolerant Antarctica Bacillus sphaericus
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000229169400002
WOS关键词	SCHIRMACHER OASIS ; SP-NOV ; BACTERIA ; ARTHROBACTER ; PROTEINS ; STRAIN ; LAKE
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/148948
作者单位	(1)Def Res & Dev Estab, Div Biotechnol, Gwalior 474002, Madhya Pradesh, India
推荐引用方式 GB/T 7714	Dhaked, RK,Alam, SI,Dixit, A,et al. Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp P9[J],2005,36(7):855-861.
APA	Dhaked, RK,Alam, SI,Dixit, A,&Singh, L.(2005).Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp P9.ENZYME AND MICROBIAL TECHNOLOGY,36(7),855-861.
MLA	Dhaked, RK,et al."Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp P9".ENZYME AND MICROBIAL TECHNOLOGY 36.7(2005):855-861.