Arid
DOI10.1016/S0168-9452(03)00220-6
Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra
Pan, ZG; Liu, CZ; Murch, SJ; El-Demerdash, M; Saxena, PK
通讯作者Saxena, PK
来源期刊PLANT SCIENCE
ISSN0168-9452
出版年2003
卷号165期号:4页码:681-687
英文摘要

Protoplast-to-plant systems for the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra have been developed. Micropropagated shoots of A. judaica and E spinosissimus were selected as plant materials suitable for protoplast isolation. The yield and viability of protoplasts in both species were greatly influenced by osmotic potential and enzyme combinations. Sustained cell division and colony formation from the protoplasts of A. Judaica were best supported by a medium containing 0.6 mol l(-1) glucose, 2.5 mumol l(-1) 6-benzylaminopurine (BAP) and 5.0 mumol l(-1) 1-naphthaleneacctic acid (NAA) in 0.6% Agarose (SeaPrep(R)) block/liquid culture. The highest frequency of colony formation in E spinosissimus was observed when the protoplasts were embedded in 0.6% Na-alginate block and cultured in a medium containing 1/2 strength Murashige and Skoog, 1962 (MS salts), Gamborg et al., 1968 (135 vitamin), 0.5 mol l(-1) glucose, 2.5 mumol l(-1) BAP and 5.0 mumol l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). The protoplast-derived colonies formed green compact calli when transferred onto 0.25% gellan gum-solidified MSO medium supplemented with 2.5 mumol l(-1) BAP and 2.5 mumol l(-1) NAA for A. judaica, and 2.5 mumol l(-1) BAP and 2.5 mumol l(-1) 2,4-D for E. spinosissimus. Shoot organogenesis from the protoplast-derived callus was induced on MS medium supplemented with 5.0 mumol l(-1) BAP for A. judaica and 2.5 mumol l(-1) BAP for E spinosissimus. The protoplast to plant regeneration protocols developed in this Study may provide the basis to investigate cell physiology and biochemistry of desert medicinal plants. (C) 2003 Elsevier Ireland Ltd. All rights reserved.


英文关键词Artemisia judaica L. Echinops spinosissimus Turra protoplasts plant regeneration Egyptian medicinal species
类型Article
语种英语
国家Canada ; Egypt
收录类别SCI-E
WOS记录号WOS:000185291000001
WOS关键词SESQUITERPENE LACTONES ; AGAROSE MEDIA ; CULTURE ; MONOSPERMA ; TOBACCO ; APPLE ; CELLS
WOS类目Biochemistry & Molecular Biology ; Plant Sciences
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/145682
作者单位(1)Univ Guelph, Dept Plant Agr, Div Biotechnol, Guelph, ON N1G 2W1, Canada;(2)Univ Mansoura, Fac Sci, Mansoura, Egypt
推荐引用方式
GB/T 7714
Pan, ZG,Liu, CZ,Murch, SJ,et al. Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra[J],2003,165(4):681-687.
APA Pan, ZG,Liu, CZ,Murch, SJ,El-Demerdash, M,&Saxena, PK.(2003).Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra.PLANT SCIENCE,165(4),681-687.
MLA Pan, ZG,et al."Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra".PLANT SCIENCE 165.4(2003):681-687.
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