Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/S0168-9452(03)00220-6 |
Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra | |
Pan, ZG; Liu, CZ; Murch, SJ; El-Demerdash, M; Saxena, PK | |
通讯作者 | Saxena, PK |
来源期刊 | PLANT SCIENCE
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ISSN | 0168-9452 |
出版年 | 2003 |
卷号 | 165期号:4页码:681-687 |
英文摘要 | Protoplast-to-plant systems for the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra have been developed. Micropropagated shoots of A. judaica and E spinosissimus were selected as plant materials suitable for protoplast isolation. The yield and viability of protoplasts in both species were greatly influenced by osmotic potential and enzyme combinations. Sustained cell division and colony formation from the protoplasts of A. Judaica were best supported by a medium containing 0.6 mol l(-1) glucose, 2.5 mumol l(-1) 6-benzylaminopurine (BAP) and 5.0 mumol l(-1) 1-naphthaleneacctic acid (NAA) in 0.6% Agarose (SeaPrep(R)) block/liquid culture. The highest frequency of colony formation in E spinosissimus was observed when the protoplasts were embedded in 0.6% Na-alginate block and cultured in a medium containing 1/2 strength Murashige and Skoog, 1962 (MS salts), Gamborg et al., 1968 (135 vitamin), 0.5 mol l(-1) glucose, 2.5 mumol l(-1) BAP and 5.0 mumol l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). The protoplast-derived colonies formed green compact calli when transferred onto 0.25% gellan gum-solidified MSO medium supplemented with 2.5 mumol l(-1) BAP and 2.5 mumol l(-1) NAA for A. judaica, and 2.5 mumol l(-1) BAP and 2.5 mumol l(-1) 2,4-D for E. spinosissimus. Shoot organogenesis from the protoplast-derived callus was induced on MS medium supplemented with 5.0 mumol l(-1) BAP for A. judaica and 2.5 mumol l(-1) BAP for E spinosissimus. The protoplast to plant regeneration protocols developed in this Study may provide the basis to investigate cell physiology and biochemistry of desert medicinal plants. (C) 2003 Elsevier Ireland Ltd. All rights reserved. |
英文关键词 | Artemisia judaica L. Echinops spinosissimus Turra protoplasts plant regeneration Egyptian medicinal species |
类型 | Article |
语种 | 英语 |
国家 | Canada ; Egypt |
收录类别 | SCI-E |
WOS记录号 | WOS:000185291000001 |
WOS关键词 | SESQUITERPENE LACTONES ; AGAROSE MEDIA ; CULTURE ; MONOSPERMA ; TOBACCO ; APPLE ; CELLS |
WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/145682 |
作者单位 | (1)Univ Guelph, Dept Plant Agr, Div Biotechnol, Guelph, ON N1G 2W1, Canada;(2)Univ Mansoura, Fac Sci, Mansoura, Egypt |
推荐引用方式 GB/T 7714 | Pan, ZG,Liu, CZ,Murch, SJ,et al. Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra[J],2003,165(4):681-687. |
APA | Pan, ZG,Liu, CZ,Murch, SJ,El-Demerdash, M,&Saxena, PK.(2003).Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra.PLANT SCIENCE,165(4),681-687. |
MLA | Pan, ZG,et al."Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra".PLANT SCIENCE 165.4(2003):681-687. |
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