Arid
Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification
Jiang, F; Gu, YX; Zheng, CD; Fan, HF
通讯作者Jiang, F
来源期刊CHINESE PHYSICS
ISSN1009-1963
出版年2003
卷号12期号:8页码:890-894
英文摘要

A new method of combining one-wavelength anomalous scattering (OAS) phasing and density modification has been described, in which the improved phases from density modification are re-introduced into OAS phasing. In this way, the phases could be improved iteratively until convergence. The OAS phasing method is based on the previously established sign-probability formula, which breaks the phase ambiguity in the OAS phasing. The implementation of this method has been available in CCP4 as OASIS. This method, although based on direct-methods, could also incorporate known phases and figures of merit into its sign-probability formula. In the implementation of OASIS, the known phases are from the positions of the anomalous scatters. In the current method, the known phases are from the density modification. The current method was tested on phasing a lysozyme crystal using anomalous scattering of sulphur atoms with diffraction data collected on an in-house x-ray source. The resulting map was well connected for the backbone atoms and clearly traceable, with an average map correlation coefficient of 0.6622 for the backbone atoms.


英文关键词direct methods sign probability anomalous scattering density modification sulphur atom phasing
类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000184547000013
WOS关键词COMBINING DIRECT-METHODS ; PROTEIN DATA-BANK ; ISOMORPHOUS REPLACEMENT ; DIFFRACTION DATA ; CRYSTALLOGRAPHY ; AMBIGUITY ; CRAMBIN
WOS类目Physics, Multidisciplinary
WOS研究方向Physics
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/144324
作者单位(1)Chinese Acad Sci, Inst Phys, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Jiang, F,Gu, YX,Zheng, CD,et al. Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification[J],2003,12(8):890-894.
APA Jiang, F,Gu, YX,Zheng, CD,&Fan, HF.(2003).Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification.CHINESE PHYSICS,12(8),890-894.
MLA Jiang, F,et al."Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification".CHINESE PHYSICS 12.8(2003):890-894.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jiang, F]的文章
[Gu, YX]的文章
[Zheng, CD]的文章
百度学术
百度学术中相似的文章
[Jiang, F]的文章
[Gu, YX]的文章
[Zheng, CD]的文章
必应学术
必应学术中相似的文章
[Jiang, F]的文章
[Gu, YX]的文章
[Zheng, CD]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。