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Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification | |
Jiang, F; Gu, YX; Zheng, CD; Fan, HF | |
通讯作者 | Jiang, F |
来源期刊 | CHINESE PHYSICS
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ISSN | 1009-1963 |
出版年 | 2003 |
卷号 | 12期号:8页码:890-894 |
英文摘要 | A new method of combining one-wavelength anomalous scattering (OAS) phasing and density modification has been described, in which the improved phases from density modification are re-introduced into OAS phasing. In this way, the phases could be improved iteratively until convergence. The OAS phasing method is based on the previously established sign-probability formula, which breaks the phase ambiguity in the OAS phasing. The implementation of this method has been available in CCP4 as OASIS. This method, although based on direct-methods, could also incorporate known phases and figures of merit into its sign-probability formula. In the implementation of OASIS, the known phases are from the positions of the anomalous scatters. In the current method, the known phases are from the density modification. The current method was tested on phasing a lysozyme crystal using anomalous scattering of sulphur atoms with diffraction data collected on an in-house x-ray source. The resulting map was well connected for the backbone atoms and clearly traceable, with an average map correlation coefficient of 0.6622 for the backbone atoms. |
英文关键词 | direct methods sign probability anomalous scattering density modification sulphur atom phasing |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000184547000013 |
WOS关键词 | COMBINING DIRECT-METHODS ; PROTEIN DATA-BANK ; ISOMORPHOUS REPLACEMENT ; DIFFRACTION DATA ; CRYSTALLOGRAPHY ; AMBIGUITY ; CRAMBIN |
WOS类目 | Physics, Multidisciplinary |
WOS研究方向 | Physics |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/144324 |
作者单位 | (1)Chinese Acad Sci, Inst Phys, Beijing 100080, Peoples R China |
推荐引用方式 GB/T 7714 | Jiang, F,Gu, YX,Zheng, CD,et al. Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification[J],2003,12(8):890-894. |
APA | Jiang, F,Gu, YX,Zheng, CD,&Fan, HF.(2003).Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification.CHINESE PHYSICS,12(8),890-894. |
MLA | Jiang, F,et al."Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification".CHINESE PHYSICS 12.8(2003):890-894. |
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