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Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis
Higashi, T; Sugitani, H; Yagi, T; Shimada, K
通讯作者Higashi, T
来源期刊BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN0918-6158
出版年2003
卷号26期号:5页码:709-711
英文摘要

Pregnenolone sulfate (PREGS) is reported to be present in higher concentration in the brain (more than 5 ng/g tissue in the rat) than in blood and is considered to be a neurosteroid. However, there are some doubts on its brain levels, because they were determined by indirect methods (e.g., GC-MS or radioimmunoassay after solvolysis). In the present study, PREGS in rat brains was determined by an enzyme-linked immunosorbent assay, which did not require solvolysis, after pretreatment with an Oasis HLB cartridge. The absolute recovery rate of PREGS through the pretreatment was 60.8%, and the quantitation limit was 33 pg/g tissue for a 200-mg of brain aliquot. Intra- and inter-assay coefficients of variation were less than 15.1 and 9.2%, respectively. The brain PREGS levels in the control rats (n=10) were less than 0.15 ng/g tissue except for one sample (0.42 ng/g tissue) and were lower than the serum levels (n=5, 0.25-0.47 ng/ml). On the contrary, the brain PREGS levels were sufficiently increased after intrapertioneal injection of 2 mg/kg body of PREGS (n=5, 0.37-1.29 ng/g tissue). These results demonstrate that, in rats, the brain PREGS may be derived from peripheral sources, and its actual levels are much lower than those previously measured by indirect methods.


英文关键词pregnenolone sulfate determination enzyme-linked immunosorbent assay neurosteroid rat brain
类型Article
语种英语
国家Japan
收录类别SCI-E
WOS记录号WOS:000182703800026
WOS关键词CHROMATOGRAPHY-MASS SPECTROMETRY ; DEHYDROEPIANDROSTERONE ; STEROIDS
WOS类目Pharmacology & Pharmacy
WOS研究方向Pharmacology & Pharmacy
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/144224
作者单位(1)Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan
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GB/T 7714
Higashi, T,Sugitani, H,Yagi, T,et al. Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis[J],2003,26(5):709-711.
APA Higashi, T,Sugitani, H,Yagi, T,&Shimada, K.(2003).Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis.BIOLOGICAL & PHARMACEUTICAL BULLETIN,26(5),709-711.
MLA Higashi, T,et al."Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis".BIOLOGICAL & PHARMACEUTICAL BULLETIN 26.5(2003):709-711.
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