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Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis | |
Higashi, T; Sugitani, H; Yagi, T; Shimada, K | |
通讯作者 | Higashi, T |
来源期刊 | BIOLOGICAL & PHARMACEUTICAL BULLETIN
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ISSN | 0918-6158 |
出版年 | 2003 |
卷号 | 26期号:5页码:709-711 |
英文摘要 | Pregnenolone sulfate (PREGS) is reported to be present in higher concentration in the brain (more than 5 ng/g tissue in the rat) than in blood and is considered to be a neurosteroid. However, there are some doubts on its brain levels, because they were determined by indirect methods (e.g., GC-MS or radioimmunoassay after solvolysis). In the present study, PREGS in rat brains was determined by an enzyme-linked immunosorbent assay, which did not require solvolysis, after pretreatment with an Oasis HLB cartridge. The absolute recovery rate of PREGS through the pretreatment was 60.8%, and the quantitation limit was 33 pg/g tissue for a 200-mg of brain aliquot. Intra- and inter-assay coefficients of variation were less than 15.1 and 9.2%, respectively. The brain PREGS levels in the control rats (n=10) were less than 0.15 ng/g tissue except for one sample (0.42 ng/g tissue) and were lower than the serum levels (n=5, 0.25-0.47 ng/ml). On the contrary, the brain PREGS levels were sufficiently increased after intrapertioneal injection of 2 mg/kg body of PREGS (n=5, 0.37-1.29 ng/g tissue). These results demonstrate that, in rats, the brain PREGS may be derived from peripheral sources, and its actual levels are much lower than those previously measured by indirect methods. |
英文关键词 | pregnenolone sulfate determination enzyme-linked immunosorbent assay neurosteroid rat brain |
类型 | Article |
语种 | 英语 |
国家 | Japan |
收录类别 | SCI-E |
WOS记录号 | WOS:000182703800026 |
WOS关键词 | CHROMATOGRAPHY-MASS SPECTROMETRY ; DEHYDROEPIANDROSTERONE ; STEROIDS |
WOS类目 | Pharmacology & Pharmacy |
WOS研究方向 | Pharmacology & Pharmacy |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/144224 |
作者单位 | (1)Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan |
推荐引用方式 GB/T 7714 | Higashi, T,Sugitani, H,Yagi, T,et al. Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis[J],2003,26(5):709-711. |
APA | Higashi, T,Sugitani, H,Yagi, T,&Shimada, K.(2003).Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis.BIOLOGICAL & PHARMACEUTICAL BULLETIN,26(5),709-711. |
MLA | Higashi, T,et al."Studies on neurosteroids XVI. Levels of pregnenolone sulfate in rat brains determined by enzyme-linked immunosorbent assay not requiring solvolysis".BIOLOGICAL & PHARMACEUTICAL BULLETIN 26.5(2003):709-711. |
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