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DOI	10.1007/s00604-002-0919-3
	Symmetry shield and XTerra reversed phase columns in HPLC determination of morphine and its metabolites
	Blahova, E; Brandsteterova, E; Netriova, J
通讯作者	Brandsteterova, E
来源期刊	MICROCHIMICA ACTA
ISSN	0026-3672
EISSN	1436-5073
出版年	2002
卷号	140 期号:3-4 页码:247-253
英文摘要	A simple HPLC method has been developed for the determination of morphine and its two metabolites, morphine-6-glucuronide and morphine-3-glucuronide in serum. An SPE procedure using OASIS cartridges was recommended for the serum sample preparation. Stationary phases of new generation (Symmetry Shield, XTerra) were tested for the HPLC separation. Mobile phases without any organic modifier and without ion pairs were applied in wide ranges of pH values (Symmetry Shield column pH = 2.5-7.2, XTerra column PH = 2.5-9.5). For higher PH values, very low contents of organic modifier could be used (1, 2%). All analytes were detected by UV detection at 210nm. Detection limits of the assay were 6.9ng/ml for morphine, 2.3ng/ml for M3G and 20.0ng/ml for M6G. The complete SPE-HPLC assay was developed for the therapeutic drug monitoring of morphine, M3G and M6G in cancer patient samples.
英文关键词	morphine HPLC stationary phases
类型	Article
语种	英语
国家	Slovakia
收录类别	SCI-E
WOS记录号	WOS:000179125700014
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; HUMAN PLASMA ; 6-GLUCURONIDE METABOLITES ; ELECTROCHEMICAL DETECTION ; HUMAN SERUM ; MORPHINE-3-GLUCURONIDE ; MORPHINE-6-GLUCURONIDE ; EXTRACTION ; GLUCURONIDES
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/143396
作者单位	(1)Fac Chem & Food Technol, Dept Analyt Chem, SK-81237 Bratislava, Slovakia;(2)Natl Inst Oncol, SK-83310 Bratislava, Slovakia
推荐引用方式 GB/T 7714	Blahova, E,Brandsteterova, E,Netriova, J. Symmetry shield and XTerra reversed phase columns in HPLC determination of morphine and its metabolites[J],2002,140(3-4):247-253.
APA	Blahova, E,Brandsteterova, E,&Netriova, J.(2002).Symmetry shield and XTerra reversed phase columns in HPLC determination of morphine and its metabolites.MICROCHIMICA ACTA,140(3-4),247-253.
MLA	Blahova, E,et al."Symmetry shield and XTerra reversed phase columns in HPLC determination of morphine and its metabolites".MICROCHIMICA ACTA 140.3-4(2002):247-253.