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	Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction
	Dobrev, PI; Kaminek, M
通讯作者	Kaminek, M
来源期刊	JOURNAL OF CHROMATOGRAPHY A
ISSN	0021-9673
出版年	2002
卷号	950 期号:1-2 页码:21-29
英文摘要	A method for separation of cytokinins from auxin and abscisic acid, which allows further separation of cytokinin ribotides from cytokinin bases, ribosides and glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and canon-exchange mode of the Oasis MCX sorbent the cationic cytokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluted by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of the method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (RIA) or HPLC-MS. (C) 2002 Elsevier Science B.V. All rights reserved.
英文关键词	solid-phase extraction cytokinins auxin abscisic acid plant hormones
类型	Article
语种	英语
国家	Czech Republic
收录类别	SCI-E
WOS记录号	WOS:000174786200002
WOS关键词	PLANT-TISSUES ; METABOLISM ; TOBACCO
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/143031
作者单位	(1)Acad Sci Czech Republ, Inst Expt Bot, CZ-16502 Prague 6, Czech Republic
推荐引用方式 GB/T 7714	Dobrev, PI,Kaminek, M. Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction[J],2002,950(1-2):21-29.
APA	Dobrev, PI,&Kaminek, M.(2002).Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction.JOURNAL OF CHROMATOGRAPHY A,950(1-2),21-29.
MLA	Dobrev, PI,et al."Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction".JOURNAL OF CHROMATOGRAPHY A 950.1-2(2002):21-29.