Arid
In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions
Juarez, MC; Passera, CB
通讯作者Juarez, MC
来源期刊BIOCELL
ISSN0327-9545
出版年2002
卷号26期号:3页码:319-324
英文摘要

The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species


Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions.


Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations. Explants were grown at 27 +/- 2degreesC, under a 16-h photoperiod. The shoots produced were used in the rooting assay using different auxin combinations. In the most efficient growth treatment, plantlets reached 100% shooting after 35 days of culture, and a mean length of 10.2 mm after 49 days of culture. A 100% rooted plantlets was obtained on a medium containing 5 mg L(-1)IBA, after 12 days of culture. Acclimatization was achieved under greenhouse conditions, showing 100% plantlet survival.


This study suggests that O. ellisiana can be successfully micropropagated by areoles, and easily acclimatizated to field conditions.


英文关键词micropropagation areole culture cold hardy forage arid forage resources
类型Article
语种英语
国家Argentina
收录类别SCI-E
WOS记录号WOS:000181033100002
WOS关键词INVITRO-PROPAGATION ; MICROPROPAGATION ; CACTACEAE ; ARGENTINA ; FORAGE
WOS类目Biology
WOS研究方向Life Sciences & Biomedicine - Other Topics
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/142178
作者单位(1)CRICYT, IADIZA, RA-5500 Mendoza, Argentina;(2)Univ Nacl Cuyo, Fac Ciencias Agrarias, RA-5505 Mendoza, Argentina
推荐引用方式
GB/T 7714
Juarez, MC,Passera, CB. In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions[J],2002,26(3):319-324.
APA Juarez, MC,&Passera, CB.(2002).In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions.BIOCELL,26(3),319-324.
MLA Juarez, MC,et al."In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions".BIOCELL 26.3(2002):319-324.
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