Knowledge Resource Center for Ecological Environment in Arid Area
In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions | |
Juarez, MC; Passera, CB | |
通讯作者 | Juarez, MC |
来源期刊 | BIOCELL
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ISSN | 0327-9545 |
出版年 | 2002 |
卷号 | 26期号:3页码:319-324 |
英文摘要 | The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions. Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations. Explants were grown at 27 +/- 2degreesC, under a 16-h photoperiod. The shoots produced were used in the rooting assay using different auxin combinations. In the most efficient growth treatment, plantlets reached 100% shooting after 35 days of culture, and a mean length of 10.2 mm after 49 days of culture. A 100% rooted plantlets was obtained on a medium containing 5 mg L(-1)IBA, after 12 days of culture. Acclimatization was achieved under greenhouse conditions, showing 100% plantlet survival. This study suggests that O. ellisiana can be successfully micropropagated by areoles, and easily acclimatizated to field conditions. |
英文关键词 | micropropagation areole culture cold hardy forage arid forage resources |
类型 | Article |
语种 | 英语 |
国家 | Argentina |
收录类别 | SCI-E |
WOS记录号 | WOS:000181033100002 |
WOS关键词 | INVITRO-PROPAGATION ; MICROPROPAGATION ; CACTACEAE ; ARGENTINA ; FORAGE |
WOS类目 | Biology |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/142178 |
作者单位 | (1)CRICYT, IADIZA, RA-5500 Mendoza, Argentina;(2)Univ Nacl Cuyo, Fac Ciencias Agrarias, RA-5505 Mendoza, Argentina |
推荐引用方式 GB/T 7714 | Juarez, MC,Passera, CB. In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions[J],2002,26(3):319-324. |
APA | Juarez, MC,&Passera, CB.(2002).In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions.BIOCELL,26(3),319-324. |
MLA | Juarez, MC,et al."In vitro propagation of Opuntia ellisiana Griff. and acclimatization to field conditions".BIOCELL 26.3(2002):319-324. |
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