Knowledge Resource Center for Ecological Environment in Arid Area
Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment | |
Melchiorre, MN; Lascano, HR; Trippi, VS | |
通讯作者 | Melchiorre, MN |
来源期刊 | BIOCELL
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ISSN | 0327-9545 |
出版年 | 2002 |
卷号 | 26期号:2页码:217-223 |
英文摘要 | Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene P-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by P-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used. |
英文关键词 | BAR bombardment GUS transformation Triticum aestivum |
类型 | Article |
语种 | 英语 |
国家 | Argentina |
收录类别 | SCI-E |
WOS记录号 | WOS:000177941400002 |
WOS关键词 | PARTICLE BOMBARDMENT ; TRITICUM-AESTIVUM ; AGROBACTERIUM-TUMEFACIENS ; GENETIC-TRANSFORMATION ; STABLE TRANSFORMATION ; RAPID PRODUCTION ; MAIZE ; EXPRESSION ; VARIETIES ; TISSUES |
WOS类目 | Biology |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/142177 |
作者单位 | (1)IFFIVE INTA, Inst Fitopatol & Fisiol Vegetal, RA-5119 Cordoba, Argentina |
推荐引用方式 GB/T 7714 | Melchiorre, MN,Lascano, HR,Trippi, VS. Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment[J],2002,26(2):217-223. |
APA | Melchiorre, MN,Lascano, HR,&Trippi, VS.(2002).Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment.BIOCELL,26(2),217-223. |
MLA | Melchiorre, MN,et al."Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment".BIOCELL 26.2(2002):217-223. |
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