Arid
Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment
Melchiorre, MN; Lascano, HR; Trippi, VS
通讯作者Melchiorre, MN
来源期刊BIOCELL
ISSN0327-9545
出版年2002
卷号26期号:2页码:217-223
英文摘要

Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene P-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by P-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.


英文关键词BAR bombardment GUS transformation Triticum aestivum
类型Article
语种英语
国家Argentina
收录类别SCI-E
WOS记录号WOS:000177941400002
WOS关键词PARTICLE BOMBARDMENT ; TRITICUM-AESTIVUM ; AGROBACTERIUM-TUMEFACIENS ; GENETIC-TRANSFORMATION ; STABLE TRANSFORMATION ; RAPID PRODUCTION ; MAIZE ; EXPRESSION ; VARIETIES ; TISSUES
WOS类目Biology
WOS研究方向Life Sciences & Biomedicine - Other Topics
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/142177
作者单位(1)IFFIVE INTA, Inst Fitopatol & Fisiol Vegetal, RA-5119 Cordoba, Argentina
推荐引用方式
GB/T 7714
Melchiorre, MN,Lascano, HR,Trippi, VS. Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment[J],2002,26(2):217-223.
APA Melchiorre, MN,Lascano, HR,&Trippi, VS.(2002).Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment.BIOCELL,26(2),217-223.
MLA Melchiorre, MN,et al."Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment".BIOCELL 26.2(2002):217-223.
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