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A determination method for estriol 3-sulfate 16-glucuronide (E-3-3S-16G) in pregnancy serum by liquid chromatography/electrospray ionization-tandem mass spectrometry has been developed. E-3-3S-16G gave an intense deprotonated ion in the negative-ion mode, which provided a product ion formed from the elimination of the glucuronic acid moiety by the collision induced dissociation.

The serum specimen was diluted, heated to remove protein followed by a solid phase extraction using an OASIS HLB cartridge, then subjected to liquid chromatography/tandem mass spectrometry. The recovery of E-3-3S-16G from serum was satisfactory and there was no interfering peak derived from the serum components at the elution position of E-3-3S-16G, but the ionization efficiency of the analyte was influenced by some substances from the serum and varied among each specimen.