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DOI | 10.1081/AL-100107525 |
Sensitive analysis of tetrodotoxin in human plasma by solid-phase extractions and gas chromatography/mass spectrometry | |
Kurono, S; Hattori, H; Suzuki, O; Yamada, T; Seno, H | |
通讯作者 | Seno, H |
来源期刊 | ANALYTICAL LETTERS
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ISSN | 0003-2719 |
出版年 | 2001 |
卷号 | 34期号:14页码:2439-2446 |
英文摘要 | A method to determine tetrodotoxin (TTX) in human plasma by solid-phase extractions and gas chromatography/mass spectrometry (GC/MS) is described. TTX in plasma samples was passed through Sep-Pak PS-2 extraction cartridges, Then the compound was converted to 2-amino-6-hydroxymethyl-8-hydroxyquinazoline (C9-base) under alkaline conditions and extracted with use of an OASIS HLB extraction cartridge. The C9-base was analyzed after trimethylsilyl derivatization by GC/MS-selected ion monitoring. Recovery rates were 35.3-50.8% and their coefficients of variation were not greater than 16.6%. The calibration curve was linear in the range of 0.5-10.0 ng ml(-1) and the detection limit was 0.1 ng ml(-1). The coefficients of within-day and day-to-day variations were not greater than 11.8%. Actual determination of TTX in rat plasma after intraperitoneal administration was also presented. |
英文关键词 | tetrodotoxin C9-base human plasma solid-phase extraction Sep-Pak PS2 OASIS HLB gas chromatography/mass spectrometry |
类型 | Article |
语种 | 英语 |
国家 | Japan |
收录类别 | SCI-E |
WOS记录号 | WOS:000172315500003 |
WOS类目 | Chemistry, Analytical |
WOS研究方向 | Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/140229 |
作者单位 | (1)Aichi Med Univ, Sch Med, Dept Legal Med, Nagakute, Aichi 4801195, Japan;(2)Hamamatsu Univ Sch Med, Dept Legal Med, Hamamatsu, Shizuoka 4313192, Japan |
推荐引用方式 GB/T 7714 | Kurono, S,Hattori, H,Suzuki, O,et al. Sensitive analysis of tetrodotoxin in human plasma by solid-phase extractions and gas chromatography/mass spectrometry[J],2001,34(14):2439-2446. |
APA | Kurono, S,Hattori, H,Suzuki, O,Yamada, T,&Seno, H.(2001).Sensitive analysis of tetrodotoxin in human plasma by solid-phase extractions and gas chromatography/mass spectrometry.ANALYTICAL LETTERS,34(14),2439-2446. |
MLA | Kurono, S,et al."Sensitive analysis of tetrodotoxin in human plasma by solid-phase extractions and gas chromatography/mass spectrometry".ANALYTICAL LETTERS 34.14(2001):2439-2446. |
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