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We examined algal flora in the aquatic system of Devils Hole, Nevada. The water is seasonally warm, near neutral in pH, and rich in dissolved carbonate, indicative of deeply circulating groundwater flowing through carbonate rock. Most algae were benthic, with only a fem planktonic representatives. Eighty-four terminal identifications in 44 genera were recorded, with diatoms and blue green algae the most speciose groups. Diatoms were major contributors to the grazing food web. Large, mat-forming filamentous algae have an important influence on the physicochemical and general structure of the benthic substrates in Del ils Hole.