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Diet and habitat selection of several species of insectivorous bats were studied in the Dead Sea area. Israel. Significant differences in diet composition were found among the species studied. Long-eared bats (Plecotus austriacus and Nycteris thebaica) tended to feed on Lepidoptera, and N. thebaica also on Diptera; Asellia tridens and Rhinolophus clivosus on Coleoptera and Hymenoptera; Rhinolophus hipposideros on Lepidoptera and Diptera; Eptesicus bottae on Hymenoptera and Lepidoptera; Pipistrellus bodenheimeri and Pipistrellus kuhlii on Diptera and Lepidoptera; and Pipistrellus rueppellii on Diptera. Dietary niche breadth (B-%V) was widest for P. bodenheimeri and narrowest for P. austriacus. There were significant species-related differences in habitat utilization. Tadarida teniotis and Rhinopoma hardwickei foraged almost exclusively in open areas: ’over water’ habitat was preferred by P. rueppellii and utilized extensively also by I? bodenheimeri; ’near street lights’ and ’edges of vegetation and cliffs’ by P. kuhlii; ’edges of vegetation and cliffs’ by Il bodenheimeri and E. bottae; and ’cluttered environment’ by A. tridens, R. hipposideros, and R. clivosus. Community structure of these bats is discussed.