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Using calli from immature zygotic embryos of Acacia tortilis subsp. raddiana, globular somatic embryos are obtained after 90 to 100 days culture in darkness on VA media added with 2,4-D (2,4 and 8 mg/l) alone or in combination with BAP (1 and 0,5 mg/l). Light and transfert on MS/2 medium containing BAP (0,5 mg/l)) and AIB (0,05 mg/l) enhance proliferation of somatic embryos. ABA (2,6 and 5,2 mg/l) or high concentration of sucrose (50 g/l allow development from globular to bipolar embryos. Development of mature somatic embryos into plantlets are obtained on MS/2 medium without hormon, but supplemented with high concentration of sucrose.