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Two new techniques have been described-one for infecting the guineapigs with schistosome cercariae and the other for recovery of blood-flukes from them with the advantage of their possible use in other animal species. For infecting the guineapigs. counted number of freshly shed cercariae of Schistosoma incognitum and S. spindale were taken in two separate small (7 cm x 15 cm) transparent LDPE polythene bags which enveloped the hind paws (tail in other animals) of the guinea-pig and held above the ground to avoid splitting of the bags.

In the perfusion technique, automatic pipetting machine has been replaced by a vertical water pump commonly used in the desert coolers. After severing the hepatic portal vein, a 16 gauze hypodermic needle (its size regulated saline flow), connected through a PVC tube With the pump, was inserted in the left ventricle of the guineapig and pump was started till liver turned pale. The perfusate was filtered through a black cotton cloth which was transferred into a petri-dish to collect the blood flukes.