Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3109/10520299809140512 |
A squash preparation method for root meristem field studies | |
Dubrovsky, JG; Contreras-Burciaga, L | |
通讯作者 | Dubrovsky, JG |
来源期刊 | BIOTECHNIC & HISTOCHEMISTRY
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ISSN | 1052-0295 |
出版年 | 1998 |
卷号 | 73期号:2页码:92-96 |
英文摘要 | We present a field method for squash preparation of root apical meristem that does not require dry ice for cell adhesion to a slide. The method is based on the use of a weak gelatin solution as a stabilizing agent to prevent cell loss. Alcian blue 8GX was used as a counterstain for better recognition of cell shapes. Root meristems of Sonoran Desert succulents were used to illustrate the method. Fixed root tips were stained by Feulgen and squashes were prepared in a 1:2 mixture of solution A (45% acetic acid) and solution B (3:1 mixture of 0.5% gelatin in water:0.02% of alcian blue 8GX in 45% acetic acid). After squashing, the material was lightly dried for 5-6 min, then dehydrated and mounted in Canada balsam. Our method permits observation of chromosomes, recognition of meristematic and nonmeristematic cells, and estimation of mitotic indices and thus recognition of growing, dormant, and dead roots. The method can be used for estimation of cell division cycle time by colchicine and other methods where squash preparation is required. |
英文关键词 | apical meristem chromosome field studies root squash succulents |
类型 | Article |
语种 | 英语 |
国家 | Mexico |
收录类别 | SCI-E |
WOS记录号 | WOS:000073479400005 |
WOS类目 | Biotechnology & Applied Microbiology ; Cell Biology |
WOS研究方向 | Biotechnology & Applied Microbiology ; Cell Biology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/135467 |
作者单位 | (1)CIBNOR, Biol Res Ctr, BCS, Div Expt Biol, Mexico City 23000, DF, Mexico |
推荐引用方式 GB/T 7714 | Dubrovsky, JG,Contreras-Burciaga, L. A squash preparation method for root meristem field studies[J],1998,73(2):92-96. |
APA | Dubrovsky, JG,&Contreras-Burciaga, L.(1998).A squash preparation method for root meristem field studies.BIOTECHNIC & HISTOCHEMISTRY,73(2),92-96. |
MLA | Dubrovsky, JG,et al."A squash preparation method for root meristem field studies".BIOTECHNIC & HISTOCHEMISTRY 73.2(1998):92-96. |
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