Arid
DOI10.3109/10520299809140512
A squash preparation method for root meristem field studies
Dubrovsky, JG; Contreras-Burciaga, L
通讯作者Dubrovsky, JG
来源期刊BIOTECHNIC & HISTOCHEMISTRY
ISSN1052-0295
出版年1998
卷号73期号:2页码:92-96
英文摘要

We present a field method for squash preparation of root apical meristem that does not require dry ice for cell adhesion to a slide. The method is based on the use of a weak gelatin solution as a stabilizing agent to prevent cell loss. Alcian blue 8GX was used as a counterstain for better recognition of cell shapes. Root meristems of Sonoran Desert succulents were used to illustrate the method. Fixed root tips were stained by Feulgen and squashes were prepared in a 1:2 mixture of solution A (45% acetic acid) and solution B (3:1 mixture of 0.5% gelatin in water:0.02% of alcian blue 8GX in 45% acetic acid). After squashing, the material was lightly dried for 5-6 min, then dehydrated and mounted in Canada balsam. Our method permits observation of chromosomes, recognition of meristematic and nonmeristematic cells, and estimation of mitotic indices and thus recognition of growing, dormant, and dead roots. The method can be used for estimation of cell division cycle time by colchicine and other methods where squash preparation is required.


英文关键词apical meristem chromosome field studies root squash succulents
类型Article
语种英语
国家Mexico
收录类别SCI-E
WOS记录号WOS:000073479400005
WOS类目Biotechnology & Applied Microbiology ; Cell Biology
WOS研究方向Biotechnology & Applied Microbiology ; Cell Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/135467
作者单位(1)CIBNOR, Biol Res Ctr, BCS, Div Expt Biol, Mexico City 23000, DF, Mexico
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GB/T 7714
Dubrovsky, JG,Contreras-Burciaga, L. A squash preparation method for root meristem field studies[J],1998,73(2):92-96.
APA Dubrovsky, JG,&Contreras-Burciaga, L.(1998).A squash preparation method for root meristem field studies.BIOTECHNIC & HISTOCHEMISTRY,73(2),92-96.
MLA Dubrovsky, JG,et al."A squash preparation method for root meristem field studies".BIOTECHNIC & HISTOCHEMISTRY 73.2(1998):92-96.
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