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Twelve ground-water samples were collected in the Nag Hammadi area, from Quaternary and Plio-Pleistocene age aquifers, The isotopic data indicate that the main recharge source of the Quaternary aquifer is the recent River Nile water, which is isotopically enriched from evaporation in Lake Nasser, Sources of recharge to the Plio-Pleistocene aquifer are: (1) Old Nile water depleted in isotope composition, which was trapped in the geological formation, characterized by low permeability and clay lenses prior to the construction of the Aswan High Dam; and (2) The leakage of palaeo water from the deep aquifer system through main faults which bisect the study area, The variation in the chemistry of ground water is thought to be related to mixing between the different water sources and leaching of fertilizers in the newly reclaimed areas in the desert fringes.