干旱区生态环境知识资源中心(Arid): Purification of a novel, heat-stable serine protease inhibitor protein from ovaries of the desert locust, Schistocerca gregaria

Arid

DOI	10.1006/bbrc.1997.7283
	Purification of a novel, heat-stable serine protease inhibitor protein from ovaries of the desert locust, Schistocerca gregaria
	Hamdaoui, A; Schoofs, L; Wateleb, S; VandenBosch, L; Verhaert, P; Waelkens, E; DeLoof, A
来源期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
EISSN	1090-2104
出版年	1997
卷号	238 期号:2 页码:357-360
英文摘要	A protease-inhibitor was isolated from mature ovaries of Schistocerca gregaria by a combination of trypsin-affinity chromatography and reverse-phase high performance liquid chromatography. It was characterized by aminoterminal amino acid sequencing using Edman degradation based automated microsequencing and by MALDI-TOF mass spectrometry, The N-terminal sequence (Y)XAEXDELA(A)EEY(Y)Q(Q)X(I)(L)M (X being a Cys, an irregular or modified amino acid) revealed no similarities with any other protease inhibitors isolated from invertebrate or vertebrate source. The 14 kDa inhibitor was found to be heat-stable. It shows potent inhibitory activity toward bovine trypsin and chymotrypsin, but not toward pancreatic elastase. It is likely that the characterized inhibitor will serve as an important tool for understanding its role in insect development. (C) 1997 Academic Press.
类型	Article
语种	英语
国家	BELGIUM ; MOROCCO
收录类别	SCI-E
WOS记录号	WOS:A1997XZ13700021
WOS关键词	HEMOLYMPH ; PEPTIDES
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/133871
作者单位	(1)UNIV CATHOLIQUE LOUVAIN, INST ZOOL, B-3000 LOUVAIN LA NEUVE, BELGIUM;(2)UNIV CADI AYYAD, FAC SCI SEMLALIA, MARRAKECH, MOROCCO;(3)KATHOLIEKE UNIV LEUVEN, FAC MED, BIOCHEM LAB, B-3001 LOUVAIN, BELGIUM
推荐引用方式 GB/T 7714	Hamdaoui, A,Schoofs, L,Wateleb, S,et al. Purification of a novel, heat-stable serine protease inhibitor protein from ovaries of the desert locust, Schistocerca gregaria[J],1997,238(2):357-360.
APA	Hamdaoui, A.,Schoofs, L.,Wateleb, S.,VandenBosch, L.,Verhaert, P.,...&DeLoof, A.(1997).Purification of a novel, heat-stable serine protease inhibitor protein from ovaries of the desert locust, Schistocerca gregaria.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,238(2),357-360.
MLA	Hamdaoui, A,et al."Purification of a novel, heat-stable serine protease inhibitor protein from ovaries of the desert locust, Schistocerca gregaria".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 238.2(1997):357-360.