干旱区生态环境知识资源中心(Arid): Kinetic properties of purified carnitine acetyltransferase from the skeletal muscle of Arabian camel (Camelus dromedarius)

Arid

DOI	10.1016/0300-9084(96)89507-1
	Kinetic properties of purified carnitine acetyltransferase from the skeletal muscle of Arabian camel (Camelus dromedarius)
	Alhomida, AS; AlJafari, AA; Duhaiman, AS; Rabbani, N; Junaid, MA
通讯作者	Alhomida, AS
来源期刊	BIOCHIMIE
ISSN	0300-9084
出版年	1996
卷号	78 期号:3 页码:204-208
英文摘要	The kinetic properties of carnitine acetyltransferase from the skeletal muscle of the Arabian camel (Camelus dromedarius) were studied. The enzyme showed an optimum pH between 7.2 and 8.2. Reciprocal plots of data obtained by varying one substrate concentration while keeping the other constant revealed lines that converged on the abscissa, indicating that the enzyme possibly follows a random mechanism of catalysis. The K(m)s for L-carnitine and acetyl-coenzyme A were 244 and 44 mu M respectively, while those for acetyl-DL-carnitine and coenzyme A (Co A)were 307 and 39 mu M respectively. The K-m for one substrate was found to be independent of the concentration of the second substrate used. Corresponding V-max values for L-CA, acetyl-Co A, acetyl DL-carnitine and Co A are 98, 98, 102 and 100 mu mol min(-1) mg(-1) protein respectively. The low K-m obtained for acetyl-DL-carnitine suggests an adaptive mechanism in this desert species for enduring prolonged dry spells without food and water.
英文关键词	carnitine acetyltransferase camel muscle kinetics random sequential mechanism
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:A1996UX41200008
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/132407
推荐引用方式 GB/T 7714	Alhomida, AS,AlJafari, AA,Duhaiman, AS,et al. Kinetic properties of purified carnitine acetyltransferase from the skeletal muscle of Arabian camel (Camelus dromedarius)[J]. King Saud University,1996,78(3):204-208.
APA	Alhomida, AS,AlJafari, AA,Duhaiman, AS,Rabbani, N,&Junaid, MA.(1996).Kinetic properties of purified carnitine acetyltransferase from the skeletal muscle of Arabian camel (Camelus dromedarius).BIOCHIMIE,78(3),204-208.
MLA	Alhomida, AS,et al."Kinetic properties of purified carnitine acetyltransferase from the skeletal muscle of Arabian camel (Camelus dromedarius)".BIOCHIMIE 78.3(1996):204-208.