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We compared transpiration estimates of three common desert landscape tree species using stem-flow gauges and lysimetry. Argentine mesquite (Prosopis alba Grisebach), desert willow [Chilopsis linearis (cav.) Sweet var. linearis], and southern live oak (Quercus virginiana Mill., seedling selection) were subjected to three irrigation regimes. Leaching fractions of +0.25, 0.00, and -0.25 were imposed for 2 years. During the summer of the second year, we conducted a comparative transpiration study. Trees growing in 190-liter plastic containers had a highly linear correlation (r = 0.98,P = 0.001) between transpiration estimated by stem-flow gauges and lysimetry. An average 18% error was measured between paired data (total runs of 14 to 72.5 hours) of stem-flow gauge and lysimetry transpiration estimates. However, a lower error was correlated significantly with longer run times (r = -0.37, P = 0.05). Based on field measurements taken in this experiment, run times would have to be >68 hours to maintain an associated error below 10%. Higher cumulative transpiration also was associated with longer run times (r = 0.80, P = 0.001). These results suggest that the stem-flow gauge can be used to estimate transpiration accurately to schedule irrigation for woody ornamental trees in an arid environment, provided that irrigation predictions are not based on short-term stem-flow gauge estimates (<68 hours).