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Restriction-fragment-length polymorphisms (RFLPs) of the sarcomeric-myosin heavy chain multigene family were studied in 13 populations of 4 chromosomal species (2n = 52,54,58 and 60) of the mole rat Spalax ehrenbergi superspecies in Israel. A minimum number of 6 hybridizing fragments occurred in the mole rat, corresponding to a minimum of 6, probably even 8, different loci of the sarcomeric myosin heavy chain. The level of.polymorphism was almost-equal-to 31%, with a low number of alleles in the polymorphic loci. All of the populations, including a desert isolate, were polymorphic at some level. Among-sample fragment variation was adequate to differentiate between some of the chromosomal species, and confirms earlier evidence that gene flow between the chromosomal species is limited.