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Multiple shoots (16 - 20 shoots per explant) were induced from cotyledonary node region of Anogeissus acuminata (Roxb. ex DC.) Guill. & Perr. on Murashige and Skoog’s (MS) medium containing IAA 0.1 mg l-1 + BAP 1.5 mg l-1 and ascorbic acid 50 mg l-1, citric acid 25.0 mg l-1, arginine 25 mg l-1 and adenine sulphate 25 mg l-1. From the first node of seedling only 4 - 6 shoots per explant were proliferated. Segments of in vitro produced shoots were used as explants for further multiplication of shoots upto 16 successive cultures at an interval of 4 week on MS medium with IAA 0.1 mg l-1 + BAP 1.0 mg l-1 and additives. The original cotyledonary explant was repeatedly subcultured upto 4 times after harvesting crop of shoots, each time. In vitro produced shoots were rooted on half strength MS medium containing 0.5 mg l-1 IBA. Plantlets were transferred to pots. Other explants (cotyledons, hypocotyl, and leaf) produced callus on medium containing auxins and cytokinins. The calluses differentiated into embryo like structures or roots on MS medium,