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An in vitro method for cloning and mass multiplication of Maytenus emarginata, a highly drought resistant tree of the Indian Desert, has been developed. Shoot segments harvested from a "plus" tree (30-year-old) were cultured to produce multiple shoots (10-15 shoots/explant) on MS medium containing 0.1 mgl-1 IAA and 2.5 mgl-1 BAP. In vitro produced shoots were cut into segments and cultured on shoot proliferation medium but with only 1.0 mgl-1 of BAP to further multiply the shoots. Isolated individual shoots were cultured on a filter paper bridge in half strength MS liquid medium containing 25 mgl-1 of IBA for 72 h in the dark at 28 +/- 2(0) C for induction of root(s). About 70-80 percent of shoots rooted. The treelets developed were hardened and transferred to pots. Around 20,000 plants can be obtained from a single explant within a period of 6 months. The protocol is highly reproducible and efficient.