Arid
DOI10.2307/1940040
POPULATION-DYNAMICS OF BACTERIOPHAGE AND BACILLUS-SUBTILIS IN SOIL
PANTASTICOCALDAS, M; DUNCAN, KE; ISTOCK, CA; BELL, JA
来源期刊ECOLOGY
ISSN0012-9658
出版年1992
卷号73期号:5页码:1888-1902
英文摘要

The dynamics of the interaction of populations of Bacillus subtilis and a temperate bacteriophage in soil microorganisms were examined. The purpose of the study was to investigate these dynamics in a structured habitat approaching that of their natural habitat in soil, in contrast to previous investigations in broth or chemostats. We addressed three main questions. What are the population dynamics of a phage-bacteria interaction in soil? What role might the heterogeneity of the soil play in shaping the interaction? Are the dynamics controlled more by the population biology of the phage or of the bacteria?


The phage used was isolated from Arizona desert soil in which B. subtilis is common. Phage and bacteria were grown separately or together in sterile soil microcosms consisting of autoclaved soil rich in organic material. Densities of phage and bacteria were estimated through repeated sterile sampling of the microcosms by spreading precise dilutions of sampled soil suspensions onto plates of microbiological media. The plates for estimation of phage densities contained in addition a lawn of host bacteria. While the principal focus is on temperate phage ecology, the dynamics of a single example of interaction between B. subtilis and virulent phage is also presented. In all cases an initial epidemic of phage occurred, followed by stable equilibria lasting weeks to months. A threshold host density for phage outbreak occurs at almost-equal-to 5 x 10(6) colony-forming units per gram of soil. At equilibrium the phage, both temperate and virulent, were much less abundant than the bacteria. The temperate phage did not depress the equilibrium host density, while the virulent phage lowered it by a factor of ten. The acidic soil of these experiments caused rapid and permanent inactivation of free phage, making the continuous interaction of phage and host essential for persistence of phage. Low levels of phage resistance (superimmunity or genetic) were typical of host populations.


Most properties of the interaction between B. subtilis and phage in soil are quite different from those observed with chemostat populations of Escherichia coli and virulent phages. Potential doubling times and rates of increase in soil for phage and B. subtilis were calculated. At equilibrium, soil slows the interaction of phage and host relative to population growth in broth culture, and possibly also makes it heterogeneous in space and time. The life-history features of B. subtilis and temperate phage are considerably more complex than those of non-sporeforming bacteria and virulent phage. The biotic relationship of temperate phage and host may be distinct from predation or parasitism. Patterns in the ecology and evolution of temperate and virulent phage were explored, leading to the expectation that temperate forms will be more common among the phages of soil bacteria.


英文关键词BACILLUS-SUBTILIS BACTERIOPHAGE BACTERIOPHAGE HOST INTERACTION LIFE HISTORIES MICROBIAL ECOLOGY MICROORGANISMS PHAGE ECOLOGY POPULATION DYNAMICS SOIL BACTERIA SOIL MICROCOSM EXPERIMENTS TEMPERATE PHAGE VIRULENT PHAGE
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:A1992JP74000037
WOS关键词LAKE WATER ; COEVOLUTION ; PHAGE ; TEMPERATE ; DENSITY ; MODEL
WOS类目Ecology
WOS研究方向Environmental Sciences & Ecology
来源机构University of Arizona
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/127539
作者单位(1)UNIV ARIZONA,DEPT NORMAL PHYSIOL,TUCSON,AZ 85721;(2)MICHIGAN STATE UNIV,CTR MICROBIAL ECOL,E LANSING,MI 48824
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PANTASTICOCALDAS, M,DUNCAN, KE,ISTOCK, CA,et al. POPULATION-DYNAMICS OF BACTERIOPHAGE AND BACILLUS-SUBTILIS IN SOIL[J]. University of Arizona,1992,73(5):1888-1902.
APA PANTASTICOCALDAS, M,DUNCAN, KE,ISTOCK, CA,&BELL, JA.(1992).POPULATION-DYNAMICS OF BACTERIOPHAGE AND BACILLUS-SUBTILIS IN SOIL.ECOLOGY,73(5),1888-1902.
MLA PANTASTICOCALDAS, M,et al."POPULATION-DYNAMICS OF BACTERIOPHAGE AND BACILLUS-SUBTILIS IN SOIL".ECOLOGY 73.5(1992):1888-1902.
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