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DOI	10.1007/s10142-022-00833-z
	Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root
	Thoppurathu, Feba Jacob; Ghorbanzadeh, Zahra; Vala, Ashish Kumar; Hamid, Rasmieh; Joshi, Meera
通讯作者	Hamid, R (corresponding author)，Agr Res Educ & Extens Org, Cotton Res Inst Iran, Dept Plant Breeding, Gorgan, Golestan, Iran.
来源期刊	FUNCTIONAL & INTEGRATIVE GENOMICS
ISSN	1438-793X
EISSN	1438-7948
出版年	2022-02
英文摘要	Peanut is one of the most valuable legumes, grown mainly in arid and semi-arid regions, where its production may be hindered by the lack of water. Therefore, breeding drought tolerant varieties is of great importance for peanut breeding programs around the world. Unlike cultivated peanuts, wild peanuts have greater genetic diversity and are an important source of alleles conferring tolerance/resistance to abiotic and biotic stresses. To decipher the transcriptome changes under drought stress, transcriptomics of roots of highly tolerant Arachis duranensis (ADU) and moderately susceptible A. stenosperma (AST) genotypes were performed. Transcriptome analysis revealed an aggregate of 1465 differentially expressed genes (DEGs), and among the identified DEGs, there were 366 single nucleotide polymorphisms (SNPs). Gene ontology and Mapman analyses revealed that the ADU genotype had a higher number of transcripts related to DNA methylation or demethylation, phytohormone signal transduction and flavonoid production, transcription factors, and responses to ethylene. The transcriptome analysis was endorsed by qRT-PCR, which showed a strong correlation value (R-2 = 0.96). Physio-biochemical analysis showed that the drought-tolerant plants produced more osmolytes, ROS phagocytes, and sugars, but less MDA, thus attenuating the effects of drought stress. In addition, three SNPs of the gene encoding transcription factor NFAY (Aradu.YE2F8), expansin alpha (Aradu.78HGD), and cytokinin dehydrogenase 1-like (Aradu.U999X) exhibited polymorphism in selected different genotypes. Such SNPs could be useful for the selection of drought-tolerant genotypes.
英文关键词	RNA-seq Peanut wild relatives Drought stress Roots qRT-PCR
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000760163500001
WOS关键词	STRESS ; EXPRESSION ; TOLERANCE ; TRAITS ; RESISTANCE ; ARACHIS ; TOPHAT
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377234
作者单位	[Thoppurathu, Feba Jacob] Kerala Agr Univ, Ctr Plant Biotechnol & Mol Biol, Trichur, India; [Ghorbanzadeh, Zahra] Agr Res Educ & Extens Org, Agr Biotechnol Res Inst Iran, Dept Syst Biol, Karaj, Iran; [Vala, Ashish Kumar; Joshi, Meera] Junagadh Agr Univ, Dept Biotechnol, Junagadh, Gujarat, India; [Hamid, Rasmieh] Agr Res Educ & Extens Org, Cotton Res Inst Iran, Dept Plant Breeding, Gorgan, Golestan, Iran
推荐引用方式 GB/T 7714	Thoppurathu, Feba Jacob,Ghorbanzadeh, Zahra,Vala, Ashish Kumar,et al. Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root[J],2022.
APA	Thoppurathu, Feba Jacob,Ghorbanzadeh, Zahra,Vala, Ashish Kumar,Hamid, Rasmieh,&Joshi, Meera.(2022).Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root.FUNCTIONAL & INTEGRATIVE GENOMICS.
MLA	Thoppurathu, Feba Jacob,et al."Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root".FUNCTIONAL & INTEGRATIVE GENOMICS (2022).