Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1007/s10142-022-00833-z |
| Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root | |
| Thoppurathu, Feba Jacob; Ghorbanzadeh, Zahra; Vala, Ashish Kumar; Hamid, Rasmieh; Joshi, Meera | |
| Corresponding Author | Hamid, R (corresponding author)，Agr Res Educ & Extens Org, Cotton Res Inst Iran, Dept Plant Breeding, Gorgan, Golestan, Iran. |
| Journal | FUNCTIONAL & INTEGRATIVE GENOMICS
 |
| ISSN | 1438-793X |
| EISSN | 1438-7948 |
| Year Published | 2022-02 |
| Abstract in English | Peanut is one of the most valuable legumes, grown mainly in arid and semi-arid regions, where its production may be hindered by the lack of water. Therefore, breeding drought tolerant varieties is of great importance for peanut breeding programs around the world. Unlike cultivated peanuts, wild peanuts have greater genetic diversity and are an important source of alleles conferring tolerance/resistance to abiotic and biotic stresses. To decipher the transcriptome changes under drought stress, transcriptomics of roots of highly tolerant Arachis duranensis (ADU) and moderately susceptible A. stenosperma (AST) genotypes were performed. Transcriptome analysis revealed an aggregate of 1465 differentially expressed genes (DEGs), and among the identified DEGs, there were 366 single nucleotide polymorphisms (SNPs). Gene ontology and Mapman analyses revealed that the ADU genotype had a higher number of transcripts related to DNA methylation or demethylation, phytohormone signal transduction and flavonoid production, transcription factors, and responses to ethylene. The transcriptome analysis was endorsed by qRT-PCR, which showed a strong correlation value (R-2 = 0.96). Physio-biochemical analysis showed that the drought-tolerant plants produced more osmolytes, ROS phagocytes, and sugars, but less MDA, thus attenuating the effects of drought stress. In addition, three SNPs of the gene encoding transcription factor NFAY (Aradu.YE2F8), expansin alpha (Aradu.78HGD), and cytokinin dehydrogenase 1-like (Aradu.U999X) exhibited polymorphism in selected different genotypes. Such SNPs could be useful for the selection of drought-tolerant genotypes. |
| Keyword in English | RNA-seq Peanut wild relatives Drought stress Roots qRT-PCR |
| Subtype | Article ; Early Access |
| Language | 英语 |
| Indexed By | SCI-E |
| WOS ID | WOS:000760163500001 |
| WOS Keyword | STRESS ; EXPRESSION ; TOLERANCE ; TRAITS ; RESISTANCE ; ARACHIS ; TOPHAT |
| WOS Subject | Genetics & Heredity |
| WOS Research Area | Genetics & Heredity |
| Document Type | 期刊论文 |
| Identifier | http://119.78.100.177/qdio/handle/2XILL650/377234 |
| Affiliation | [Thoppurathu, Feba Jacob] Kerala Agr Univ, Ctr Plant Biotechnol & Mol Biol, Trichur, India; [Ghorbanzadeh, Zahra] Agr Res Educ & Extens Org, Agr Biotechnol Res Inst Iran, Dept Syst Biol, Karaj, Iran; [Vala, Ashish Kumar; Joshi, Meera] Junagadh Agr Univ, Dept Biotechnol, Junagadh, Gujarat, India; [Hamid, Rasmieh] Agr Res Educ & Extens Org, Cotton Res Inst Iran, Dept Plant Breeding, Gorgan, Golestan, Iran |
| Recommended Citation GB/T 7714 | Thoppurathu, Feba Jacob,Ghorbanzadeh, Zahra,Vala, Ashish Kumar,et al. Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root[J],2022. |
| APA | Thoppurathu, Feba Jacob,Ghorbanzadeh, Zahra,Vala, Ashish Kumar,Hamid, Rasmieh,&Joshi, Meera.(2022).Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root.FUNCTIONAL & INTEGRATIVE GENOMICS. |
| MLA | Thoppurathu, Feba Jacob,et al."Unravelling the treasure trove of drought-responsive genes in wild-type peanut through transcriptomics and physiological analyses of root".FUNCTIONAL & INTEGRATIVE GENOMICS (2022). |
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