干旱区生态环境知识资源中心(Arid): A targeted bioinformatics approach identifies highly variable cell surface proteins that are unique to Glomeromycotina

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DOI	10.1007/s00572-021-01066-x
	A targeted bioinformatics approach identifies highly variable cell surface proteins that are unique to Glomeromycotina
	Schultz, Carolyn J.; Wu, Yue; Baumann, Ute
通讯作者	Schultz, CJ (corresponding author)，Univ Adelaide, Sch Agr Food & Wine, Waite Res Inst, Adelaide, SA, Australia.
来源期刊	MYCORRHIZA
ISSN	0940-6360
EISSN	1432-1890
出版年	2022
卷号	32 期号:1 页码:45-66
英文摘要	Diversity in arbuscular mycorrhizal fungi (AMF) contributes to biodiversity and resilience in natural environments and healthy agricultural systems. Functional complementarity exists among species of AMF in symbiosis with their plant hosts, but the molecular basis of this is not known. We hypothesise this is in part due to the difficulties that current sequence assembly methodologies have assembling sequences for intrinsically disordered proteins (IDPs) due to their low sequence complexity. IDPs are potential candidates for functional complementarity because they often exist as extended (non-globular) proteins providing additional amino acids for molecular interactions. Rhizophagus irregularis arabinogalactan-protein-like proteins (AGLs) are small secreted IDPs with no known orthologues in AMF or other fungi. We developed a targeted bioinformatics approach to identify highly variable AGLs/IDPs in RNA-sequence datasets. The approach includes a modified multiple k-mer assembly approach (Oases) to identify candidate sequences, followed by targeted sequence capture and assembly (mirabait-mira). All AMF species analysed, including the ancestral family Paraglomeraceae, have small families of proteins rich in disorder promoting amino acids such as proline and glycine, or glycine and asparagine. Glycine- and asparagine-rich proteins also were found in Geosiphon pyriformis (an obligate symbiont of a cyanobacterium), from the same subphylum (Glomeromycotina) as AMF. The sequence diversity of AGLs likely translates to functional diversity, based on predicted physical properties of tandem repeats (elastic, amyloid, or interchangeable) and their broad pI ranges. We envisage that AGLs/IDPs could contribute to functional complementarity in AMF through processes such as self-recognition, retention of nutrients, soil stability, and water movement.
英文关键词	Arbuscular mycorrhizal fungi Proline- and glycine-rich Glycosylphosphatidylinositol (GPI)-anchored proteins Tandem repeats Intrinsically disordered proteins (IDPs) Glomeromycotina
类型	Article
语种	英语
开放获取类型	Green Published, hybrid
收录类别	SCI-E
WOS记录号	WOS:000742582700001
WOS关键词	ARBUSCULAR MYCORRHIZAL FUNGI ; GLOMUS-INTRARADICES ; GENOME-WIDE ; PLANT ; EVOLUTION ; CLASSIFICATION ; SEQUENCES ; DISORDER ; GLOMALIN ; PROLINE
WOS类目	Plant Sciences ; Mycology
WOS研究方向	Plant Sciences ; Mycology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376557
作者单位	[Schultz, Carolyn J.; Wu, Yue; Baumann, Ute] Univ Adelaide, Sch Agr Food & Wine, Waite Res Inst, Adelaide, SA, Australia
推荐引用方式 GB/T 7714	Schultz, Carolyn J.,Wu, Yue,Baumann, Ute. A targeted bioinformatics approach identifies highly variable cell surface proteins that are unique to Glomeromycotina[J],2022,32(1):45-66.
APA	Schultz, Carolyn J.,Wu, Yue,&Baumann, Ute.(2022).A targeted bioinformatics approach identifies highly variable cell surface proteins that are unique to Glomeromycotina.MYCORRHIZA,32(1),45-66.
MLA	Schultz, Carolyn J.,et al."A targeted bioinformatics approach identifies highly variable cell surface proteins that are unique to Glomeromycotina".MYCORRHIZA 32.1(2022):45-66.