干旱区生态环境知识资源中心(Arid): Genetic Variation in the Prion Protein Gene (PRNP) of Two Tunisian Goat Populations

Arid

DOI	10.3390/ani11061635
	Genetic Variation in the Prion Protein Gene (PRNP) of Two Tunisian Goat Populations
	Kdidi, Samia; Yahyaoui, Mohamed Habib; Conte, Michela; Chiappini, Barbara; Hammadi, Mohamed; Khorchani, Touhami; Vaccari, Gabriele
通讯作者	Kdidi, S (corresponding author), Univ Gabes, Inst Reg Arides, Livestock & Wildlife Lab, Route El Djorf,Km 22-5, Medenine 4119, Tunisia.
来源期刊	ANIMALS
ISSN	2076-2615
出版年	2021
卷号	11 期号:6
英文摘要	Simple Summary Goat production is contributing to the economic and social development of rural areas in arid lands, within harsh conditions of Southern Tunisia. In this geographic zone, there are two caprine populations: the native goat population and the crossed goat population. Genotyping goats for the prion protein gene (PRNP) allows us to estimate their level of genetic susceptibility to scrapie disease. In the present work, the Sanger sequencing method of the entire PRNP coding sequence was used to determine the different PRNP genotypes and haplotypes in two populations (116 animals). This study represents the first investigation on goats' PRNP genetic variability in Tunisia, and the results are useful in the design of national breeding programs. Scrapie is a fatal prion disease. It belongs to transmissible spongiform encephalopathies (TSEs), and occurs in sheep and goats. Similarly, to ovine species, the prion protein gene (PRNP) plays a major role in conferring resistance or susceptibility to TSE in goats. This study assesses the variability of PRNP in native and crossed-breed goat populations raised in the Southeast of Tunisia and provides information on the distribution of PRNP haplotypes and genotypes in these goat populations. A total of 116 unrelated goats including 82 native and 34 crossed-breed goats were screened for PRNP polymorphisms using Sanger sequencing. Sequence analysis revealed 10 non-synonymous polymorphisms (G37V, M137I, R139S, I142M, H143R, N146D, R154H, R211Q, Q222K, and S240P), giving rise to 12 haplotypes and 23 genotypes. Moreover, four silent mutations were detected at codons 30, 42, 138, and 179; the former was reported for the first time in goat (nucleotide 60 c -> t). Interestingly, the PrP variants associated with resistance (D146 and K222) or with a prolonged incubation time of goat to scrapie (M142, R143, H154, Q211) were absent or detected with low frequencies except for H154 variant, which is present with high frequency (1%, 1%, 4%, 0%, 88%, and 6%, respectively, for native goats, and 0%, 1%, 0%, 1%, 78%, and 1%, respectively, for crossed goats). The analysis of PRNP polymorphisms of goats raised in other regions of the country will be useful in getting a global view of PRNP genetic variability and the feasibility of goat breeding programs in Tunisia.
英文关键词	PRNP polymorphism goat South-East Tunisia
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000665446900001
WOS关键词	CAPRINE PRP GENE ; CYPRUS GOATS ; CODON 146 ; SCRAPIE ; POLYMORPHISMS ; RESISTANCE ; ASSOCIATION ; IDENTIFICATION ; VARIABILITY ; INFECTION
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS研究方向	Agriculture ; Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/349461
作者单位	[Kdidi, Samia; Yahyaoui, Mohamed Habib; Hammadi, Mohamed; Khorchani, Touhami] Univ Gabes, Inst Reg Arides, Livestock & Wildlife Lab, Route El Djorf,Km 22-5, Medenine 4119, Tunisia; [Conte, Michela; Chiappini, Barbara; Vaccari, Gabriele] Ist Super Sanita, Dept Food Safety Nutr & Vet Publ Hlth, Viale Regina Elena 299, I-00161 Rome, Italy
推荐引用方式 GB/T 7714	Kdidi, Samia,Yahyaoui, Mohamed Habib,Conte, Michela,et al. Genetic Variation in the Prion Protein Gene (PRNP) of Two Tunisian Goat Populations[J],2021,11(6).
APA	Kdidi, Samia.,Yahyaoui, Mohamed Habib.,Conte, Michela.,Chiappini, Barbara.,Hammadi, Mohamed.,...&Vaccari, Gabriele.(2021).Genetic Variation in the Prion Protein Gene (PRNP) of Two Tunisian Goat Populations.ANIMALS,11(6).
MLA	Kdidi, Samia,et al."Genetic Variation in the Prion Protein Gene (PRNP) of Two Tunisian Goat Populations".ANIMALS 11.6(2021).