干旱区生态环境知识资源中心(Arid): Limited Microcystin, Anatoxin and Cylindrospermopsin Production by Cyanobacteria from Microbial Mats in Cold Deserts

Arid

DOI	10.3390/toxins12040244
	Limited Microcystin, Anatoxin and Cylindrospermopsin Production by Cyanobacteria from Microbial Mats in Cold Deserts
	Khomutovska, Nataliia; Sandzewicz, Malgorzata; Lach, Lukasz; Suska-Malawska, Malgorzata; Chmielewska, Monika; Mazur-Marzec, Hanna; Ceglowska, Marta; Niyatbekov, Toirbek; Wood, Susanna A.; Puddick, Jonathan; Kwiatowski, Jan; Jasser, Iwona
通讯作者	Jasser, I (corresponding author), Univ Warsaw, Biol & Chem Res Ctr, Fac Biol, Inst Environm Biol, Zwirki & Wigury 101, PL-02089 Warsaw, Poland.
会议名称	11th International Conference on Toxic Cyanobacteria (ICTC)
会议日期	MAY 05-10, 2019
会议地点	Krakow, POLAND
英文摘要	Toxic metabolites are produced by many cyanobacterial species. There are limited data on toxigenic benthic, mat-forming cyanobacteria, and information on toxic cyanobacteria from Central Asia is even more scarce. In the present study, we examined cyanobacterial diversity and community structure, the presence of genes involved in toxin production and the occurrence of cyanotoxins in cyanobacterial mats from small water bodies in a cold high-mountain desert of Eastern Pamir. Diversity was explored using amplicon-based sequencing targeting the V3-V4 region of the 16S rRNA gene, toxin potential using PCR-based methods (mcy, nda, ana, sxt), and toxins by enzyme-linked immunosorbent assays (ELISAs) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Molecular identification of cyanobacteria showed a high similarity of abundant taxa to Nostoc PCC-73102, Nostoc PCC-7524, Nodularia PCC-935 and Leptolyngbya CYN68. The PCRs revealed the presence of mcyE and/or ndaF genes in 11 samples and mcyD in six. The partial sequences of the mcyE gene showed high sequence similarity to Nostoc, Planktothrix and uncultured cyanobacteria. LC-MS/MS analysis identified six microcystin congeners in two samples and unknown peptides in one. These results suggest that, in this extreme environment, cyanobacteria do not commonly produce microcystins, anatoxins and cylindrospermopsins, despite the high diversity and widespread occurrence of potentially toxic taxa.
英文关键词	cyanotoxins microbial mats V3-V4 hypervariable region 16S rRNA gene mcyA mcyE mcyD ndaF anaC sxtA potential toxin producers
来源出版物	TOXINS
EISSN	2072-6651
出版年	2020
卷号	12
期号	4
出版者	MDPI
类型	Article; Proceedings Paper
语种	英语
开放获取类型	Green Published, gold
收录类别	CPCI-S
WOS记录号	WOS:000533393900060
WOS关键词	IDENTIFICATION ; CYANOTOXINS ; DIVERSITY ; HEPATOTOXIN ; INHIBITION ; EVOLUTION ; ACCURATE ; ECOLOGY ; BLOOMS ; TOXIN
WOS类目	Food Science & Technology ; Toxicology
WOS研究方向	Food Science & Technology ; Toxicology
资源类型	会议论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/337010
作者单位	[Khomutovska, Nataliia; Sandzewicz, Malgorzata; Lach, Lukasz; Suska-Malawska, Malgorzata; Chmielewska, Monika; Kwiatowski, Jan; Jasser, Iwona] Univ Warsaw, Biol & Chem Res Ctr, Fac Biol, Inst Environm Biol, Zwirki & Wigury 101, PL-02089 Warsaw, Poland; [Mazur-Marzec, Hanna] Univ Gdansk, Inst Oceanog, Div Marine Biotechnol, Marszalka Pilsudskiego 46 Ave, PL-81378 Gdynia, Poland; [Ceglowska, Marta] Polish Acad Sci, Inst Oceanol, Dept Chem & Biochem, Powstancow Warszawy 55, PL-81712 Sopot, Poland; [Niyatbekov, Toirbek] Acad Sci Republ Tajikistan, Inst Bot Plant Physiol & Genet, 27 Karamov Str, Dushanbe 734017, Tajikistan; [Wood, Susanna A.; Puddick, Jonathan] Cawthron Inst, Private Bag 2, Nelson 7042, New Zealand
推荐引用方式 GB/T 7714	Khomutovska, Nataliia,Sandzewicz, Malgorzata,Lach, Lukasz,et al. Limited Microcystin, Anatoxin and Cylindrospermopsin Production by Cyanobacteria from Microbial Mats in Cold Deserts[C]:MDPI,2020.