干旱区生态环境知识资源中心(Arid): Surveillance and Characterization of Drug-Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years' Period

Arid

DOI	10.4103/ijmy.ijmy_94_19
	Surveillance and Characterization of Drug-Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years' Period
	Rocio Imperiale, Belen; Beatriz Di Giulio, Angela; Belen Mancino, Maria; Jose Zumarraga, Martin; Susana Morcillo, Nora
通讯作者	Imperiale, BR
来源期刊	INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY
ISSN	2212-5531
出版年	2019
卷号	8 期号:3 页码:223-228
英文摘要	Background: Argentina is considered a country with a middle tuberculosis (TB) incidence. However, according to the last national epidemiological report released in 2018, since 2013, the trends are steadily increasing. The aims of this study were to determine the drug-resistance (DR), multi-DR and extensively DR (MDR/XDR-TB), and rifampicin resistance (RIF-R) burden as a part of the local TB diagnosis (June 2010-August 2018); to detect the mutations associated to isoniazid (INH) and RIF-R and their geographical distribution; arid to analyze the lineage relationship among the genetic patterns of the isolates circulating in the community. Methods: Respiratory and extrapulmonary specimens were processed by Ziehl-Neelsen stain and cultured on specific media. Drug-susceptibility testing of isolates was performed by the MGIT 960 and a colorimetric micro-method. Mutations conferring DR were detected by Genotype and DNA sequencing. Results: The study showed a DR-TB prevalence of approximately 20% of the isolated strains, while M/XDR-TB-and particularly RIF-R-affected more than 5.0% of the total amount of cases. DR geographical distribution revealed isolates carrying mutations in the inhA gene promoter region only constrained to three districts where it was also registered two same family relatives' cases with the infrequent rpoB 5522 L/Q mutation. The fact that most DR/MDR-TB isolates were not grouped in genetic clusters suggested that these cases may mostly have occurred due to endogenous reactivation rather than recently transmission. Conclusion: According to the obtained results, it would be convenient, in highly MDR-TB suspected individuals, to confirm phenotypically, the INH and RIF susceptibility detected by molecular tests.
英文关键词	Drug-resistance genotyping mutations tuberculosis
类型	Article
语种	英语
开放获取类型	DOAJ Gold, Green Published
收录类别	ESCI
WOS记录号	WOS:000487704300003
WOS关键词	RAPID DETECTION ; COMPLEX ; ASSAY ; TRANSMISSION ; CHALLENGES ; 1ST
WOS类目	Infectious Diseases ; Microbiology
WOS研究方向	Infectious Diseases ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/333824
作者单位	[Rocio Imperiale, Belen] Consejo Nacl Invest Cient & Tecn, Inst Expt Med, Lab Immunol Resp Dis, Natl Acad Med, Buenos Aires, DF, Argentina; [Beatriz Di Giulio, Angela] Petrona V de Cordero Hosp, Mycobacteriol Lab, San Fernando, Buenos Aires, Argentina; [Belen Mancino, Maria; Susana Morcillo, Nora] Dr Cetrangolo Hosp, TB & Mycobacterioses Lab, Vicente Lopez, Argentina; [Jose Zumarraga, Martin] CONICET INTA, Inst Agrobiotechnol & Mol Biol IABIMO, Hurlingham, Buenos Aires, Argentina
推荐引用方式 GB/T 7714	Rocio Imperiale, Belen,Beatriz Di Giulio, Angela,Belen Mancino, Maria,et al. Surveillance and Characterization of Drug-Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years' Period[J],2019,8(3):223-228.
APA	Rocio Imperiale, Belen,Beatriz Di Giulio, Angela,Belen Mancino, Maria,Jose Zumarraga, Martin,&Susana Morcillo, Nora.(2019).Surveillance and Characterization of Drug-Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years' Period.INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY,8(3),223-228.
MLA	Rocio Imperiale, Belen,et al."Surveillance and Characterization of Drug-Resistant Mycobacterium tuberculosis Isolated in a Reference Hospital from Argentina during 8 Years' Period".INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY 8.3(2019):223-228.