干旱区生态环境知识资源中心(Arid): Synchrotron infrared imaging of advanced glycation endproducts (AGEs) in cardiac tissue from mice fed high glycemic diets

Arid

DOI	10.3233/BSI-130057
	Synchrotron infrared imaging of advanced glycation endproducts (AGEs) in cardiac tissue from mice fed high glycemic diets
	Birarda, Giovanni; Holman, Elizabeth A.; Fu, Shang; Weikel, Karen; Hu, Ping; Blankenberg, Francis G.; Holman, Hoi-Ying; Taylor, Allen
通讯作者	Holman, HY
来源期刊	BIOMEDICAL SPECTROSCOPY AND IMAGING
ISSN	2212-8794
EISSN	2212-8808
出版年	2013
卷号	2 期号:4 页码:301-315
英文摘要	Recent research findings correlate an increased risk for dieases such as diabetes, macular degeneration and cardiovascular disease (CVD) with diets that rapidly raise the blood sugar levels; these diets are known as high glycemic index (GI) diets which include white breads, sodas and sweet deserts. Lower glycemia diets are usually rich in fruits, non-starchy vegetables and whole grain products. The goal of our study was to compare and contrast the effects of a low vs. high glycemic diet using the biochemical composition and microstructure of the heart. The improved spatial resolution and signal-to-noise for SR-FTIR obtained through the coupling of the bright synchrotron infrared photon source to an infrared spectral microscope enabled the molecular-level observation of diet-related changes within unfixed fresh frozen histologic sections of mouse cardiac tissue. High and low glycemic index (GI) diets were started at the age of five-months and continued for one year, with the diets only differing in their starch distribution (high GI diet = 100% amylopectin versus low GI diet = 30% amylopectin/70% amylose). Serial cryosections of cardiac tissue for SR-FTIR imaging alternated with adjacent hematoxylin and eosin (H&E) stained sections allowed not only fine-scale chemical analyses of glycogen and glycolipid accumulation along a vein as well as protein glycation hotspots co-localizing with collagen cold spots but also the tracking of morphological differences occurring in tandem with these chemical changes. As a result of the bright synchrotron infrared photon source coupling, we were able to provide significant molecular evidence for a positive correlation between protein glycation and collagen degradation in our mouse model. Our results bring a new insight not only to the effects of long-term GI dietary practices of the public but also to the molecular and chemical foundation behind the cardiovascular disease pathogenesis commonly seen in diabetic patients.
英文关键词	AGE CVD diabetes fluorescence glycemia glycation infrared N center dot-(carboxymethyl) lysine PAGE pentosidine RAGE sugar
类型	Article
语种	英语
开放获取类型	Bronze, Green Accepted
收录类别	ESCI
WOS记录号	WOS:000214729500005
WOS关键词	END-PRODUCTS AGES ; OXIDATIVE STRESS ; CARDIOVASCULAR-DISEASE ; MACULAR DEGENERATION ; LIPID-PEROXIDATION ; CROSS-LINKING ; HEART-DISEASE ; DIABETIC-RAT ; MOUSE MODEL ; COLLAGEN
WOS类目	Spectroscopy
WOS研究方向	Spectroscopy
来源机构	University of California, Berkeley
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/330303
作者单位	[Birarda, Giovanni; Hu, Ping; Holman, Hoi-Ying] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley Synchrotron Infrared Struct Biol Program, Berkeley, CA 94720 USA; [Holman, Elizabeth A.; Blankenberg, Francis G.] Dept Radiol & Pediat, Mol Imaging Program, Stanford, CA USA; [Fu, Shang; Weikel, Karen; Taylor, Allen] Tufts Univ, Jean Mayer USDA HNRCA Tufts Univ, Lab Nutr & Vis Res, Boston, MA USA; [Weikel, Karen] Boston Univ, Boston Med Ctr, Sch Med, Boston, MA 02118 USA
推荐引用方式 GB/T 7714	Birarda, Giovanni,Holman, Elizabeth A.,Fu, Shang,et al. Synchrotron infrared imaging of advanced glycation endproducts (AGEs) in cardiac tissue from mice fed high glycemic diets[J]. University of California, Berkeley,2013,2(4):301-315.
APA	Birarda, Giovanni.,Holman, Elizabeth A..,Fu, Shang.,Weikel, Karen.,Hu, Ping.,...&Taylor, Allen.(2013).Synchrotron infrared imaging of advanced glycation endproducts (AGEs) in cardiac tissue from mice fed high glycemic diets.BIOMEDICAL SPECTROSCOPY AND IMAGING,2(4),301-315.
MLA	Birarda, Giovanni,et al."Synchrotron infrared imaging of advanced glycation endproducts (AGEs) in cardiac tissue from mice fed high glycemic diets".BIOMEDICAL SPECTROSCOPY AND IMAGING 2.4(2013):301-315.