干旱区生态环境知识资源中心(Arid): Effects of Incubation of Human Brain Microvascular Endothelial Cells and Astrocytes with Pyridostigmine Bromide, DEET, or Permethrin in the Absence or Presence of Metal Salts

Arid

DOI	10.3390/ijerph17228336
	Effects of Incubation of Human Brain Microvascular Endothelial Cells and Astrocytes with Pyridostigmine Bromide, DEET, or Permethrin in the Absence or Presence of Metal Salts
	Hoffman, Jessica F.; Kalinich, John F.
通讯作者	Kalinich, JF
来源期刊	INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
EISSN	1660-4601
出版年	2020
卷号	17 期号:22
英文摘要	Gulf War Illness (GWI) is a chronic, multi-symptom illness suffered by over one-third of American military veterans who served in the Persian Gulf War between 1990 and 1991. No current single-exposure scenario accounts for all the symptoms observed in GWI, and instead may be due to a multi-exposure scenario. As a larger effort to understand how one category of multi-exposure scenarios of organic compounds such as nerve gas prophylactic pyridostigmine bromide, or insecticides/pesticides such as N,N-diethyl-m-toluamide (DEET) and permethrin, plus heavy metals found in inhaled dust particles (Al, Fe, Ni, Sr, DU, Co, Cu, Mn, and Zn) might play a role in neural aspects of GWI, we begin this initial study to examine the toxicity and oxidative damage markers of human brain endothelial cell and human astrocyte cell cultures in response to these compounds. A battery of cytotoxicity assessments, including the MTT assay, Neutral Red uptake, and direct microscopic observation, was used to determine a non-toxic dose of the test compounds. After testing a wide range of doses of each compound, we chose a sub-toxic dose of 10 mu M for the three organic compounds and 1 mu M for the nine metals of interest for co-exposure experiments on cell cultures and examined an array of oxidative stress-response markers including nitric oxide production, formation of protein carbonyls, production of thiobarbituric acid-reactive substances, and expression of proteins involved in oxidative stress and cell damage. Many markers were not significantly altered, but we report a significant increase in nitric oxide after exposure to any of the three compounds in conjunction with depleted uranium.
英文关键词	Gulf War Illness pyridostigmine bromide permethrin DEET desert dust metal toxicity
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000594942100001
WOS关键词	AL-ESKAN-DISEASE ; N,N-DIETHYL-M-TOLUAMIDE DEET ; DERMAL APPLICATION ; INSECT REPELLENT ; BARRIER ; EXPOSURE ; URANIUM ; HEALTH ; NEUROTOXICITY ; STRESS
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/327807
作者单位	[Hoffman, Jessica F.; Kalinich, John F.] Uniformed Serv Univ Hlth Sci, Armed Forces Radiobiol Res Inst, Internal Contaminat & Met Toxic Program, Bethesda, MD 20889 USA
推荐引用方式 GB/T 7714	Hoffman, Jessica F.,Kalinich, John F.. Effects of Incubation of Human Brain Microvascular Endothelial Cells and Astrocytes with Pyridostigmine Bromide, DEET, or Permethrin in the Absence or Presence of Metal Salts[J],2020,17(22).
APA	Hoffman, Jessica F.,&Kalinich, John F..(2020).Effects of Incubation of Human Brain Microvascular Endothelial Cells and Astrocytes with Pyridostigmine Bromide, DEET, or Permethrin in the Absence or Presence of Metal Salts.INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH,17(22).
MLA	Hoffman, Jessica F.,et al."Effects of Incubation of Human Brain Microvascular Endothelial Cells and Astrocytes with Pyridostigmine Bromide, DEET, or Permethrin in the Absence or Presence of Metal Salts".INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 17.22(2020).