干旱区生态环境知识资源中心(Arid): Identifying error and accurately interpreting environmental DNA metabarcoding results: A case study to detect vertebrates at arid zone waterholes

Arid

DOI	10.1111/1755-0998.13170
	Identifying error and accurately interpreting environmental DNA metabarcoding results: A case study to detect vertebrates at arid zone waterholes
	Furlan, Elise M.; Davis, Jenny; Duncan, Richard P.
通讯作者	Furlan, EM
来源期刊	MOLECULAR ECOLOGY RESOURCES
ISSN	1755-098X
EISSN	1755-0998
出版年	2020
卷号	20 期号:5 页码:1259-1276
英文摘要	Environmental DNA (eDNA) metabarcoding surveys enable rapid, noninvasive identification of taxa from trace samples with wide-ranging applications from characterizing local biodiversity to identifying food-web interactions. However, the technique is prone to error from two major sources: (a) contamination through foreign DNA entering the workflow, and (b) misidentification of DNA within the workflow. Both types of error have the potential to obscure true taxon presence or to increase taxonomic richness by incorrectly identifying taxa as present at sample sites, but multiple error sources can remain unaccounted for in metabarcoding studies. Here, we use data from an eDNA metabarcoding study designed to detect vertebrate species at waterholes in Australia's arid zone to illustrate where and how in the workflow errors can arise, and how to mitigate those errors. We detected the DNA of 36 taxa spanning 34 families, 19 orders and five vertebrate classes in water samples from waterholes, demonstrating the potential for eDNA metabarcoding surveys to provide rapid, noninvasive detection in remote locations, and to widely sample taxonomic diversity from aquatic through to terrestrial taxa. However, we initially identified 152 taxa in the samples, meaning there were many false positive detections. We identified the sources of these errors, allowing us to design a stepwise process to detect and remove error, and provide a template to minimize similar errors that are likely to arise in other metabarcoding studies. Our findings suggest eDNA metabarcoding surveys need to be carefully conducted and screened for errors to ensure their accuracy.
英文关键词	arid zone biodiversity eDNA metabarcoding false detection false positive terrestrial fauna type I error
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000571480100010
WOS关键词	BIODIVERSITY ASSESSMENT ; BUFFER ZONES ; PCR ; SENSITIVITY ; DIVERSITY ; INSIGHTS ; BARCODES ; HOLOCENE ; SPACE
WOS类目	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/326411
作者单位	[Furlan, Elise M.; Duncan, Richard P.] Univ Canberra, Inst Appl Ecol, Bruce, ACT, Australia; [Davis, Jenny] Charles Darwin Univ, Res Inst Environm & Livelihoods, Coll Engn IT & Environm, Casuarina, NT, Australia
推荐引用方式 GB/T 7714	Furlan, Elise M.,Davis, Jenny,Duncan, Richard P.. Identifying error and accurately interpreting environmental DNA metabarcoding results: A case study to detect vertebrates at arid zone waterholes[J],2020,20(5):1259-1276.
APA	Furlan, Elise M.,Davis, Jenny,&Duncan, Richard P..(2020).Identifying error and accurately interpreting environmental DNA metabarcoding results: A case study to detect vertebrates at arid zone waterholes.MOLECULAR ECOLOGY RESOURCES,20(5),1259-1276.
MLA	Furlan, Elise M.,et al."Identifying error and accurately interpreting environmental DNA metabarcoding results: A case study to detect vertebrates at arid zone waterholes".MOLECULAR ECOLOGY RESOURCES 20.5(2020):1259-1276.