干旱区生态环境知识资源中心(Arid): Identification and Distribution of NBS-Encoding Resistance Genes of Dactylis glomerata L. and Its Expression Under Abiotic and Biotic Stress

Arid

DOI	10.1007/s10528-020-09977-8
	Identification and Distribution of NBS-Encoding Resistance Genes of Dactylis glomerata L. and Its Expression Under Abiotic and Biotic Stress
	Ren, Shuping 1; Sun, Min 2; Yan, Haidong 2; Wu, Bingchao 2; Jing, Tingting 2; Huang, Linkai 2; Zeng, Bing 1
通讯作者	Huang, Linkai ; Zeng, Bing
来源期刊	BIOCHEMICAL GENETICS
ISSN	0006-2928
EISSN	1573-4927
出版年	2020
卷号	58 期号:6 页码:824-847
英文摘要	Orchardgrass (Dactylis glomerata L.) is drought resistant and tolerant to barren landscapes, making it one of the most important forages for animal husbandry, as well as ecological restoration of rocky landscapes that are undergoing desertification. However, orchardgrass is susceptible to rust, which can significantly reduce its yield and quality. Therefore, understanding the genes that underlie resistance against rust in orchardgrass is critical. The evolution, cloning of plant disease resistance genes, and the analysis of pathogenic bacteria induced expression patterns are important contents in the study of interaction between microorganisms and plants. Genes with nucleotide binding site (NBS) structure are disease-resistant genes ubiquitous in plants and play an important role in plant attacks against various pathogens. Using sequence analysis and re-annotation, we identified 413 NBS resistance genes in orchardgrass. Similar to previous studies, NBS resistance genes containing TIR (toll/interleukin-1 receptor) domain were not found in orchardgrass. The NBS resistance genes can be divided into four types: NBS (up to 264 homologous genes, accounting for 64% of the total number of NBS genes in orchardgrass), NBS-LRR, CC-NBS, and CC-NBS-LRR (minimum of 26 homologous genes, only 6% of the total number of NBS genes in orchardgrass). These 413 NBS resistance genes were unevenly distributed across seven chromosomes where chromosome 5 had up to 99 NBS resistance genes. There were 224 (54%) NBS resistance genes expressed in different tissues (roots, stems, leaves, flowers, and spikes), and we did not detect expression for 45 genes (11%). The remaining 145 (35%) were expressed in some tissues. And we found that 11 NBS resistance genes were differentially expressed under waterlogging stress, 5 NBS resistance genes were differentially expressed under waterlogging and drought stress, and 1 NBS resistance was is differentially expressed under waterlogging and heat stress. Most importantly, we found that 65 NBS resistance genes were significantly expressed in different control groups. On the 7th day of inoculation, 23 NBS resistance genes were differentially expressed in high resistance materials alone, of which 7 NBS resistance genes regulate the plant-pathogen interaction pathway by encoding RPM1. At the same time, 2 NBS resistance genes that were differentially expressed in the high resistance material after inoculation were also differentially expressed in abiotic stress. In summary, the NBS resistance gene plays a crucial role in the resistance of orchardgrass to rust.
英文关键词	Orchardgrass NBS resistance genes RNA-seq Abiotic stress Rust stress
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000538367800001
WOS关键词	GENOME-WIDE IDENTIFICATION ; RUST PUCCINIA-GRAMINIS ; DISEASE-RESISTANCE ; LRR GENE ; RNA-SEQ ; ORCHARDGRASS ; WHEAT ; DROUGHT ; FAMILY ; LEAF
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/319301
作者单位	1.Southwest Univ, Coll Anim Sci, Rongchang Campus, Chongqing 402460, Peoples R China; 2.Sichuan Agr Univ, Fac Anim Sci & Technol, Dept Grassland Sci, Chengdu 611130, Peoples R China
推荐引用方式 GB/T 7714	Ren, Shuping,Sun, Min,Yan, Haidong,et al. Identification and Distribution of NBS-Encoding Resistance Genes of Dactylis glomerata L. and Its Expression Under Abiotic and Biotic Stress[J],2020,58(6):824-847.
APA	Ren, Shuping.,Sun, Min.,Yan, Haidong.,Wu, Bingchao.,Jing, Tingting.,...&Zeng, Bing.(2020).Identification and Distribution of NBS-Encoding Resistance Genes of Dactylis glomerata L. and Its Expression Under Abiotic and Biotic Stress.BIOCHEMICAL GENETICS,58(6),824-847.
MLA	Ren, Shuping,et al."Identification and Distribution of NBS-Encoding Resistance Genes of Dactylis glomerata L. and Its Expression Under Abiotic and Biotic Stress".BIOCHEMICAL GENETICS 58.6(2020):824-847.