干旱区生态环境知识资源中心(Arid): Non-invasive measurement of metabolic rates in wild, free-living birds using doubly labelled water

Arid

DOI	10.1111/1365-2435.13230
	Non-invasive measurement of metabolic rates in wild, free-living birds using doubly labelled water
	Bourne, Amanda R.1; McKechnie, Andrew E.2,3; Cunningham, Susan J.1; Ridley, Amanda R.1,4; Woodborne, Stephan M.5,6; Karasov, William H.7
通讯作者	Bourne, Amanda R.
来源期刊	FUNCTIONAL ECOLOGY
ISSN	0269-8463
EISSN	1365-2435
出版年	2019
卷号	33 期号:1 页码:162-174
英文摘要	Doubly labelled water (DLW) is routinely used to measure energy expenditure and water turnover in free-ranging animals. Standard methods involve capture, blood sampling for baseline measurement, injection with isotopic tracers, captivity for an equilibration period, post-dose blood sampling, release and subsequent recapture for final blood sampling. Single sampling methods that minimise disturbance by reducing capture and handling time have been developed and tested. Sampling faeces rather than blood could further reduce disturbance to study animals in a range of species and study systems. However, the extent to which estimates of metabolic rate derived from blood and faecal samples diverge has not been investigated. We compared isotopic enrichment in blood and faecal samples taken concurrently from captive Southern Pied Babblers Turdoides bicolor. Isotopic enrichment levels in faeces and in blood were used to calculate initial and final ratios of delta 18O/delta 2H for each individual. We then used these ratios to calculate daily energy expenditure (DEE) and directly compared measurements from blood samples with those from faecal samples within individuals. We found that faecal sampling resulted in estimates of DEE that agree with those based on blood sampling. Additionally, we field-tested a faecal sampling protocol with a habituated population of babblers in the southern Kalahari Desert. During the field test, study animals were not captured or handled for either dosing or sampling. Field-testing confirmed the practical feasibility of non-invasive dosing and sampling techniques in free-living animals, and we obtained measurements of DEE that we used to test an a priori prediction that DEE is inversely related to air temperature. Our data show decreasing DEE with increasing air temperature, a pattern consistent with studies testing similar predictions in birds using traditional DLW methods. We demonstrate that faecal samples can substitute for blood when measuring DEE using DLW and provide a method that will allow field-based researchers to obtain sound physiological measurements while minimising handling and removal of study animals from their natural environments.
英文关键词	avian physiology daily energy expenditure ecological energetics metabolic rate non-invasive doubly labelled water pied babbler
类型	Article
语种	英语
国家	South Africa ; Australia ; USA
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000456691600015
WOS关键词	DAILY ENERGY-EXPENDITURE ; CO2 PRODUCTION ; FORAGING BEHAVIOR ; POTENTIAL ERRORS ; FIELD ; VALIDATION ; ENERGETICS ; TURNOVER ; ANIMALS ; FEMALE
WOS类目	Ecology
WOS研究方向	Environmental Sciences & Ecology
来源机构	University of Western Australia
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/215843
作者单位	1.Univ Cape Town, DST NRF Ctr Excellence, FitzPatrick Inst African Ornithol, Rondebosch, South Africa; 2.Univ Pretoria, Dept Zool & Entomol, Fitzpatrick Inst, DST NRF Ctr Excellence, Hatfield, South Africa; 3.South African Natl Biodivers Inst, Natl Zool Garden, Pretoria, South Africa; 4.Univ Western Australia, Sch Biol Sci, Ctr Evolutionary Biol, Crawley, Australia; 5.iThemba LABS, Johannesburg, South Africa; 6.Univ Pretoria, Dept Zool & Entomol, Mammal Res Inst, Hatfield, South Africa; 7.Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI USA
推荐引用方式 GB/T 7714	Bourne, Amanda R.,McKechnie, Andrew E.,Cunningham, Susan J.,et al. Non-invasive measurement of metabolic rates in wild, free-living birds using doubly labelled water[J]. University of Western Australia,2019,33(1):162-174.
APA	Bourne, Amanda R.,McKechnie, Andrew E.,Cunningham, Susan J.,Ridley, Amanda R.,Woodborne, Stephan M.,&Karasov, William H..(2019).Non-invasive measurement of metabolic rates in wild, free-living birds using doubly labelled water.FUNCTIONAL ECOLOGY,33(1),162-174.
MLA	Bourne, Amanda R.,et al."Non-invasive measurement of metabolic rates in wild, free-living birds using doubly labelled water".FUNCTIONAL ECOLOGY 33.1(2019):162-174.