干旱区生态环境知识资源中心(Arid): Cyanobacterial diversity in mat sample obtained from hypersaline desert, Rann of Kachchh

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DOI	10.1007/s13205-019-1837-y
	Cyanobacterial diversity in mat sample obtained from hypersaline desert, Rann of Kachchh
	Patel, Hiral M.1; Rastogi, Rajesh P.1,2; Trivedi, Ujjval 1; Madamwar, Datta 1
通讯作者	Patel, Hiral M.
来源期刊	3 BIOTECH
ISSN	2190-572X
EISSN	2190-5738
出版年	2019
卷号	9 期号:8
英文摘要	Rann of Kachchh (RoK) is a unique geoformation, which is exposed to dynamic environmental changes such as salinity, temperature, and nutrients throughout the year. In this study, the pooled mat sample was examined for the cyanobacterial community structure using culture-dependent and culture-independent approaches. Taxonomic profiling was studied using amplicon sequencing that revealed the enrichment of Pseudanabaenales and Oscillatoriales by QIIME and MG-RAST, respectively. Other abundant orders were represented by Chroococcales, Nostocales, and unclassified cyanobacteria by both approaches. Nine cyanobacterial cultures were isolated from mat samples showing 90-98% similarities with available sequences in GenBank. The culture-dependent study suggested that mat was dominated by cyanobacterial orders such as Oscillatoriales-filamentous and Chroococcales-unicellular. Our results from the culture-dependent approach also indicated that despite high similarities in gene sequences, six cyanobacteria fall into the separate clade in the phylogenetic analysis that could be signs of evolution due to an extreme environment. Cultured isolates are correlated well with abundant taxa from amplicon sequencing. Further, protein profiling was done specifically for phycobiliproteins which will be helpful to elucidate their roles in light harvesting and energy transfer mechanism in the unique environment of RoK.
英文关键词	Cyanobacteria Microbial mat Diversity Amplicon sequencing Evolution of cyanobacteria
类型	Article
语种	英语
国家	India
开放获取类型	Green Published
收录类别	SCI-E
WOS记录号	WOS:000477004300001
WOS关键词	COMMUNITY ; BACTERIAL ; PHOTOSYNTHESIS ; PHYLOGENY ; CRUSTS ; HOT ; DNA
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/213822
作者单位	1.Sardar Patel Univ, PG Dept Biosci, Satellite Campus,Vadtal Rd, Bakrol 388315, Gujarat, India; 2.Indira Pariyavaran Bhavan, Minist Environm Forest & Climate Change, New Delhi 110003, India
推荐引用方式 GB/T 7714	Patel, Hiral M.,Rastogi, Rajesh P.,Trivedi, Ujjval,et al. Cyanobacterial diversity in mat sample obtained from hypersaline desert, Rann of Kachchh[J],2019,9(8).
APA	Patel, Hiral M.,Rastogi, Rajesh P.,Trivedi, Ujjval,&Madamwar, Datta.(2019).Cyanobacterial diversity in mat sample obtained from hypersaline desert, Rann of Kachchh.3 BIOTECH,9(8).
MLA	Patel, Hiral M.,et al."Cyanobacterial diversity in mat sample obtained from hypersaline desert, Rann of Kachchh".3 BIOTECH 9.8(2019).