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New records of 20 reptile species and subspecies on the Red Sea coast of Sinai Peninsula and Eastern Desert in Egypt are given. A zoogeographical analyses of the herpetofauna and comparison of the species compositions results to estimate the biodiversity for each subregion of Eastern Egypt. The faunal richness, taxonomic diversity and endemism level in hyperarid deserts of the Egypt are discussed. Mountainous South Sinai and southern part of the South Eastern Desert is suggested to consider as biodiversity hotspots in Eastern Egypt.